Canine leishmaniosis is a disease caused by Leishmania infantum, a vector-borne parasite. Due to the zoonotic potential of canine leishmaniosis, infected dogs must be identified. Serological assays are the most common methods for the detection of L. infantum infection in dogs used in veterinary practice. The aim of the study was to assess the performance of a rapid immunochromatographic test ( FAST est LEISH ® , MEGACOR Diagnostik) for the detection of specific antibodies to that of the L. infantum in dog sera. The results were simultaneously compared using a commercial brand of indirect immunofluorescence antibody test and an in-house enzyme-linked immunosorbent assay as references. Between the two reference tests, 232 serum samples out of 244, produced concordant results while 12 exhibited discordant results. Of the 232 concordant samples, 121 were classified as L. infantum seropositive, and 111 samples were previously classified as L. infantum seronegative by a combination of the reference assays. All samples that were seropositive by the reference tests were also positive according to the rapid test, and only one sample that was seronegative according to the two reference assays was positive according to the rapid test. Compared with the reference tests, the rapid test sensitivity was 100%, specificity was 99.1%, accuracy was 99.6%, Cohen’s kappa coefficient was 0.99, and the area under receiver operating characteristic curve was 0.995. The FAST est LEISH ® is a rapid, qualitative in-clinic test with high sensitivity and specificity. Electronic supplementary material The online version of this article (10.1186/s13028-019-0473-1) contains supplementary material, which is available to authorized users.

Resumen: En la actualidad, existen en la Unión Europea distintos sistemas de alta velocidad ferroviaria. La ma or densidad de población y el ma or equilibrio de sus redes urbanas, han permitiJo a Italia y Alemania dotarse de servicios de alta velocidad que, al contrario de lo que sucede en Francia y España, se inscriben de manera armoniosa dentro del conjunto de la red ferroviaria. Por otra parte, después de más de quince años de la puesta en marcha de la primera línea de alta velocidad en Europa, ya pueden empezar a extraerse las primeras conclusiones en lo referente al impacto socioeconómico que tiene este modo de transporte en el medio urbano. Si bien las ciudades servidas por el T.A.V. (Tren de Alta Velocidad), experimentan una mejora general de su accesibilidad, estas ven a l mismo tiem o degradarse sus servicios de tren convencional y avión en a uellas líneas don& el T.A.V. ejerce su competencia. La llegada de la alta velocidal, suele ser aprovechada para remodelar urbanísticamente el barrio de la estación, zona en general, muy degradada. Por otra parte, la presencia del T.A.V. no suele ser considerada por las em resas de terciario superior como la principal causa para localizarse en el barrio 2' e la estación, si bien es un factor m tenido en cuenta por aquellas empresas que ardan relación con otros 11~x0s urbanos que se encuentran en la línea alta velocidad. Por lo que res ecta al turismo, la P posibilidad de hacer el viaje de ida y vuelta en el mismo día, a ecta negativamente a l número de pernoctas registradas en la ciudad. El turismo de negocios y de congresos se ve, en cambio muy beneficiado.Palabras clave: Accesibilidad, barrio de la estación, ciudad, España, Francia, localización empresarial, Tren de Alta Velocidad, turismo, Unión Europea.Abstract: Nowadays, different High Speed Train systems exists in the European Union. Italy and Germany have made good use of their I-iigh population densities and their balanced urban nehvorks for having High Speed services that fit harmouniosely whithin the whole train network. After more than 15 years of High Speed Trains in Europe, the first social and economic conclussions about the urban impact of this new way of transport can be seen. Cities served by T.G.V. show a general improvement m their accessibility. However, this rnay be to the detriment of traditional train and plane services in routes where they are in direct competition with the TGV. In general, councils rnake the most of the arrival of High S eed Trains for assisting the recovery of degraded areas like station quarters. d e presence of the T.G.V. is not considered, by quaternary sector companies, as the most important reason to locate in the area surrounding the station. However, this is an important consideration for companies