Objective:used traditionally by the Ibibio people of Southern Nigeria for the Heterotis rotundifolia (Sm.) Jacq. Fel treatment of pain, stomach ulcer, malaria and other inflammatory diseases . was evaluated for antiulcer activity Materials and Methods: In this study, the effects of the extract (300-900 mg/kg) and different fractions [n-hexane (HEX), ethyl acetate (EAC), methanol (MET) and aqueous (AQU), 600 mg/kg) on experimentally induced ulcer were studied in rats using ethanol, indomethacin and histamine -induced ulcer models.: 300-900 Results The extract ( mg/kg) inhibited ethanol, indomethacin and histamine -induced ulcer models in a dose dependent fashion. The various degrees of inhibitions were statistically significant (p<0.05, 0.01, 0.001). The effect of the extract and the different fractions were comparable to that of the standard drugs the MET fraction having the highest activity used, . GC-MS analysis of the MET extract revealed the of presence polyunsaturated fatty acids such as tridecanoic acid-12methyl ester, -5,8,11,14,17-Eicosapentaenoic acid, octadecanoic acid methyl ester, tetracosanoic acid, 9,12-cis Octadecadienoic acid (Z,Z)-, and hexadecanoic acid ethyl ester. : Conclusion Thus, demonstrated a H. rotundifolia good antiulcer activity which supports the use of this plant among the Ibibio people of Southern Nigeria ethnopharmacology for ulcer treatment.