Preeclampsia, a disorder with a heterogeneous physiopathology, can be attributed to maternal, fetal, and/or placental factors. Long non-coding RNAs (lncRNAs) refer to a class of non-coding RNAs, the essential regulators of biological processes; their differential expression has been associated with the pathogenesis of multiple diseases. The study aimed to identify lncRNAs, expressed in the placentas and plasma of patients who presented with preeclampsia, as potential putative biomarkers of the disease. In silico analysis was performed to determine lncRNAs differentially expressed in the placentas of patients with preeclampsia, using a previously published RNA-Seq dataset. Seven placentas and maternal plasma samples collected at delivery from preterm preeclamptic patients (≤37 gestational weeks of gestation), and controls were used to validate the expression of lncRNAs by qRT-PCR. Six lncRNAs were validated and differentially expressed (p < 0.05) in the preeclampsia and control placentas: UCA1 and HCG4 were found upregulated, and LOC101927355, LINC00551, PART1, and NRAD1 downregulated. Two of these lncRNAs, HCG4 and LOC101927355, were also detected in maternal plasma, the latter showing a significant decrease (p = 0.03) in preeclamptic patients compared to the control group. In silico analyses showed the cytoplasmic location of LOC101927355, which suggests a role in post-transcriptional gene regulation. The detection of LOC101927355 in the placenta and plasma opens new possibilities for understanding the pathogenesis of preeclampsia and for its potential use as a biomarker.

Se describe el caso clínico de un paciente canino, hembra de 2 años, raza French Poodle, quien había presentado tres celos evidentes en un periodo menor a seis meses, según refiere el propietario. En la exploración clínica inicial se observó un paciente apoplético y en examen de aparato genitourinario se evidenció abundante secreción cero-sanguinolenta vaginal. El estudio ecográfico de la región inguinal revela la presencia de una estructura hiperecogénica de forma redondeada no específica con múltiples áreas oncogénicas sugestivas de piometra. Se practicó citología por punción de la masa guiada por ecógrafo que arrojó como diagnóstico presuntivo neoplasia de células redondas. Se practica laparotomía exploratoria en la que se evidenció una masa de color rojizo en el cérvix de 8 cm de diámetro y 3 cm de ancho. El estudio histopatológico de la muestra de la masa tumoral dio como diagnóstico definitivo neoplasia maligna de células grandes consistente con linfoma no Hodgkin de grado intermedio. La confrontación con biomarcadores CD3 arrojó un resultado de reactividad severa con distribución multifocal extensa. Dadas las condiciones de casuística se realizó la eutanasia. La necropsia dejó en evidencia focos metastásicos en costilla.

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