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Ever increasing rate in the production of automotive vehicles and the significance of decreasing the pollutions in nature trigger numerous researches to find the alternative solution. Biodiesel has been considered a vital fuel for diesel engine view point. In the present study a single cylinder diesel engine was run fully on non-petroleum fuels blends. In this regard Jatropha methyl ester (JME) and waste transformer oil (WTO) were selected as a fuel for the replacement of diesel. diesel engines such as fuel direct injection method is used. The WTO at low percentages (10-40% at regular intervals of 10% on a volume basis), was blended with diesel, to get the fuel blends for the investigation. The performance and emission terms were compared, analyzed and presented in this article.