No abstract

Crude porcine pancreatic lipase was puriÐed by removing waterinsoluble impurities (residual lipid material) associated with it by a conventional method, using hydrotropic additives and by liquid coacervate extraction. Maximum yield with good recovery of activity was obtained when hydrotropes were used to separate the associated lipids from lipase. The thermostability of the enzyme was also checked in the solutions of additives such as sodium butyl monoglycol sulfate (Na-BMGS), proline and Triton X-114. In Na-BMGS solutions above a concentration of 0É2 mol dm~3 the lipase activity decreased beyond 50¡C whereas in 1 mol dm~3 proline solution it was retained even at 80¡C, showing a good thermostabilizing e †ect. However, in the presence of Triton X-114 the enzyme was completely inactivated with increase in temperature.1998 SCI. ( J. Chem. T echnol. Biotechnol. 71, 155È161 (1998)

Aims Intraoperative 3D navigation (ION) allows high accuracy to be achieved in spinal surgery, but poor workflow has prevented its widespread uptake. The technical demands on ION when used in patients with adolescent idiopathic scoliosis (AIS) are higher than for other more established indications. Lean principles have been applied to industry and to health care with good effects. While ensuring optimal accuracy of instrumentation and safety, the implementation of ION and its associated productivity was evaluated in this study for AIS surgery in order to enhance the workflow of this technique. The aim was to optimize the use of ION by the application of lean principles in AIS surgery. Methods A total of 20 consecutive patients with AIS were treated with ION corrective spinal surgery. Both qualitative and quantitative analysis was performed with real-time modifications. Operating time, scan time, dose length product (measure of CT radiation exposure), use of fluoroscopy, the influence of the reference frame, blood loss, and neuromonitoring were assessed. Results The greatest gains in productivity were in avoiding repeat intraoperative scans (a mean of 248 minutes for patients who had two scans, and a mean 180 minutes for those who had a single scan). Optimizing accuracy was the biggest factor influencing this, which was reliant on incremental changes to the operating setup and technique. Conclusion The application of lean principles to the introduction of ION for AIS surgery helps assimilate this method into the environment of the operating theatre. Data and stakeholder analysis identified a reproducible technique for using ION for AIS surgery, reducing operating time, and radiation exposure. Cite this article: Bone Joint J. 2020;102-B(1):5–10

: Crude porcine pancreatic lipase was puriÐed by removing waterinsoluble impurities (residual lipid material) associated with it by a conventional method, using hydrotropic additives and by liquid coacervate extraction. Maximum yield with good recovery of activity was obtained when hydrotropes were used to separate the associated lipids from lipase. The thermostability of the enzyme was also checked in the solutions of additives such as sodium butyl monoglycol sulfate (Na-BMGS), proline and Triton X-114. In Na-BMGS solutions above a concentration of 0É2 mol dm~3 the lipase activity decreased beyond 50¡C whereas in 1 mol dm~3 proline solution it was retained even at 80¡C, showing a good thermostabilizing e †ect. However, in the presence of Triton X-114 the enzyme was completely inactivated with increase in temperature.1998 SCI. ( J. Chem. T echnol. Biotechnol. 71, 155È161 (1998)

Background: To present the first known reported case of late onset idiopathic scoliosis with concomitant neural anomalies, treated with sequential distraction using magnetic growth rod, had significant improvement in both cranio-cervical and intraspinal anomaly.Methods: A caucasian, growing female child (at the age of ten) presented with moderately progressive late onset right thoracic scoliosis. She was found to have Chiari type I malformation and a cervicothoracic syrinx on routine pre-operative MRI scanning. We treated this child by inserting magnetic growing rod (MGR) system. After 48 months of follow up with serial distractions, the metalwork (MGR) was removed due to aseptic wound breakdown and granuloma formation. Subsequently due to the progression of deformity, a definitive posterior instrumented spinal fusion was done. A repeat MRI Scan of the Spine was done prior to this definitive procedure to assess for any residual neural anomalies.Results: The Chiari type I malformation appeared to have completely resolved, with no cerebellar tonsillar herniation seen, and a significant improvement in the size of the cervicothoracic syringomyelia effectively downgrading it to a prominent central canal.Conclusions: This is a unique case of progressive late onset idiopathic scoliosis with associated Chiari malformation and syringomyelia, showing an improvement in these neural anomalies after gradual and protracted distractive lengthening of the spine with MGR.