Aim : Methodology :Results : Interpretation :Varietal identity and their purity assessment is essential to assert intellectual rights to breeder, helps seed testing and certification agencies to ensure pure seeds to the farmers, buyers, processors and consumers.In the present study, molecular characterization and genetic purity analysis of nine indica rice hybrids and respective 13 parental lines (22 genotypes) were done utilizing grow out test grow out test and micro-satellite markers based DNA fingerprinting approach. A total of 48 microsatellite (SSR and STMS) markers spanning over whole rice genome were screened.he statistical analysis of grow out test revealed that 88.25 per cent genetic purity was assessed in 3 hybrids tested. Twelve out of 48 microsatellite used were found polymorphic and amplified a total of 29 alleles among the genotypes with an average polymorphic information content value of 0.45. Out of 12 informative markers, nine were found polymorphic across the genotypes and produced unique fingerprints. Five STMS markers (RM154, RM164, RM234, RM258 and RM519) showed polymorphism over all the genotypes and were identified as referral marker for use in unambiguous identification and protection of nine hybrids. Nevertheless, marker RM 19 amplified allele was specific to the parental lines of CNRH 3 and APHR 2 (having common CMS line, IR62829). The results on genetic purity test through GOT had highly significant positive correlation (r=0.96) with molecular marker analysis.Genotype specific STMS markers identified in the study and their close association with the ability of grow out test, emphasizes immense scope for molecular approaches t o b e a n a l t e r n a t i v e s i n unambiguous assessment of genetic purity of hybrids and their parents. The molecular markers based genetic purity assessment may serve as a probable substitute to grow out test, which is a c u m b e r s o m e a n d t i m e consuming task and would benefit the farmers and agencies involved in this venture, hybrid rice seed production and cultivation.