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Deep eutectic solvent comprising of choline chloride and D(+)-glucose was synthesized by mixing in the molar ratio 2:1. Iron oxide nanoparticles were successfully synthesized by co-precipitation method. Deep eutectic solvent consisting of D(+)-glucose acts as a promising reducing agent for the synthesis of iron oxide nanoparticles. Direct optical band gap of iron oxide nanoparticles was found to be 2.262 eV. Powder X-ray diffraction technique was used to identify the crystalline phases. Surface morphology analysis by Field emission scanning electron microscopy and high resolution transmission electron microscopy confirmed the rod shape structure of the iron oxide nanoparticles. Selected area electron diffraction pattern revealed the identity of lattice planes with the XRD data. The antibacterial and photocatalytic activities of the iron oxide nanoparticles were also studied. The synthesized iron oxide nanoparticles showed appreciable antibacterial and photocatalytic activities