Gene amplification requires a biological sample which is collected prior to the demands. The sample for such analysis plays a vital role as they serve as a resource for the core material-Deoxyribonucleic acid. A sample for gene amplification or any analysis would be collected prior to the demands and type of analysis. Human blood has been an essential resource of DNA from the commencement of DNA extraction in the 19th century. After then several protocols developed according to the requirement of both samples of different forms and their retrieval as various forms and methods. The sample has various characteristics and prerequisites when collected for gene analysis. Some important characteristics of sample collection methods are significant, which are not widely taken. This work analyses various general sample collection protocols and highlights some of the major characteristics and prerequisites for the sample. This work initiates and delivers to attain the core of genomics by bringing out the basic raw materials importance and consequences on amplification which is highly sensitive.

Green revolution initiatives occurred between the 1930s and the late 1960s around the world, notably by Norman Ernest Borlaug, Cresco, Lowa, US. born.Later transformed to India by Mankombu Sambasivan Swaminathan, who was born in Kumbakonam, Tamil Nadu, IN. He was one who known as the father of the green revolution, also the back bone for green research findings of high-yield varieties of wheat and rice in India. Also, administrative contributions towards various green initiations. Today in the era of computing will deliver growth in various fields, including industries called Industry 4.0 in, similar to that Information and Communication Technology obtain development in various fields, which directly and indirectly related to our agriculture. Even Agriculture needs to be getting various up-gradation by which not simply by hardware and software. The modern scientific societies state that the era of developing software applications is not enough for the next generation growth, it needs technology growth for today's findings. As same Identifying and incorporating, those innovative technologies based scientific findings for delivering the solution for next generation green revolution is the most challenging issue today.