In the present study the ethanolic extract of Aquilaria malaccensis (Agar wood) leaves before inoculation (BF) and after inoculation (AF)) was evaluated for its anticancer potentials. The leaf extracts (BF and AF) was subjected to in-vitro cytotoxicity studies on EAC cells, normal spleen cells and DLA cells. The leaf extracts showed dose dependent cytotoxicity with increase in dose. The IC50 value for BF and the AF leaf extract was 79.09μg/ml and 79.20μg/ml respectively. The effect on primary spleen cells was observed to be more than 100μg/ml. The study concluded that the extract of the said plant leaves has significant potential anticancer activity. All these results clearly indicate that the crude extract has a remarkable capacity to inhibit the growth of Ascites tumor induced by EAC cell line in animals.