The present study deals with description of a new species of Pseudophyllidean Cestode genus Ptychobothrium, Loennberg, 1889 collected from intestine of freshwater fish, Mastacembelus armatus (Lacepede, 1800) at Mahur, Dist. Nanded M.S. India during February, 2011 to January, 2013. Ptychobothrium vitellaris Sp.Nov. comes closer to all known species of genus Ptychobothrium, Loennberg, 1889 in general topography of organs but differs due to sessile Scolex, paired bothria, apical disk at anterior end of scolex, Neck absent, Mature Proglottids broader than long, Cirrus pouch pyriform, Testes 40-45 in numbers, Ovary bilobed and Vitellaria follicular.
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