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ABSTRACT. It has been shown that diets containing polyunsaturated omega-3 fatty acids may modulate the immune system. Paracoccidioidomycosis (PCM) is a systemic mycosis of great importance in Latin America, caused by the thermo-dimorphic fungus Paracoccidioides brasiliensis. The aim of this study was to evaluate the influence of n-3 PUFAs in lymphoproliferative activity in murine experimental PCM. Spleen lymphocytes from animals infected with an isolate of P. brasiliensis (Pb18) and fed with diets supplemented with flaxseed oil or a commercial diet for eight weeks, were obtained and cultured for evaluation of lymphoproliferative activity by MTT assay. In uninfected animals and those fed an enriched-flaxseed diet the evaluated lymphoproliferative activity was inhibited in the first week; however, this activity resumed at later stages. On the other hand, in animals infected and fed an enriched-flaxseed diet, the interaction between the stimulus given by the presence of the fungus and the action of n-3 PUFAs appeared to stimulate lymphocyte proliferation after the first week of infection. n-3 PUFAs are shown to influence lymphoproliferative activity of mice in experimental PCM.Keywords: omega-3, paracoccidioidomycosis, imune cell.Influência do ácido graxo poli-insaturado n-3 na atividade proliferativa de linfócitos durante a infecção com Paracoccidioides brasiliensis RESUMO. Tem-se demonstrado que dietas contendo ácido graxo poli-insaturado ômega-3 podem modular o sistema imune. A Paracoccidioidomicose é uma micose sistêmica de grande importância na América Latina, causada pelo fungo termo-dimórfico Paracoccidioides brasiliensis. O objetivo do estudo foi avaliar a influência do AGPI n-3 na atividade linfoproliferativa na PCM experimental murina. Linfócitos esplênicos de animais infectados com o isolado de P. brasiliensis e alimentados com uma dieta suplementada com óleo de linhaça por 8 semanas, foram obtidos e cultivados para avaliação da atividade linfoproliferativa através do ensaio de MTT. Nos animais não infectados e alimentados com a dieta enriquecida com linhaça houve a inibição da atividade linfoproliferativa na primeira semana, sendo retomada no estágio mais tardio. Por outro lado, nos animais infectados e suplementados com a dieta com óleo de linhaça, a interação entre o estímulo dado pela presença do fungo e a ação do AGPI n-3 parecem ter estimulado a proliferação de linfócitos depois da primeira semana de infecção. AGPI n-3 podem ter influenciado a atividade linfoproliferativa dos camundongos na PCM experimental.Palavras-chave: ômega-3, paracoccidioidomicose, células imune.

Collaborative drug therapy management in primary health care involves communication among the physician, pharmacist and user of simvastatin and can result in safer results regarding patient wellbeing. The aim of the study was to investigate muscle adverse events and risk factors related to simvastatin. For patients who developed muscle adverse events, collaborative drug therapy management was performed in an attempt to resolve the symptoms. A non-randomized case study was conducted at the single basic health unit in the city of Peabiru, Parana, Brazil, for a period of one year. Patients were interviewed using a structured form. To confirm muscle adverse events, the patient was referred to a physician and submitted to the suspension and return to treatment. Thyroid-stimulating hormone, creatine kinase and alanine aminotransferase exams were performed. A sample of 148 users of simvastatin was selected. Eleven patients had some type of simvastatinassociated muscle adverse event (myopathy), among whom seven had muscle symptoms (myalgia) and four had elevated creatine kinase, but were asymptomatic (asymptomatic myopathy). Collaborative drug therapy management focused on simvastatin for five patients with myalgia led to improvements in the quality of life of two patients. EVENTOS ADVERSOS MUSCULARES ENTRE USUÁRIOS

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