The study of Indonesian's marine sponge Callyspongia Sp. against white spot syndrome virus has been conducted in June 2017. Bioactive metabolite terpene isolated based on methods of bioassay-guided separation. LCMS-ESI spectrum indicate that molecular formula C 24 H 44 O 5 with molecular weight is 412.576 m/z. FTIR spectrum indicated bioactive metabolite has ether C-O group at 1261 cm -1 and hydroxy group O-H at 3419 cm -1 . The spectrum of 1 H NMR and 13 C NMR indicated that metabolite active compound as polyhydroxy isocopalane. The antiviral activity of isolate compound has been conducted against white spot syndrome virus from Litopenaeus vannamei. The result antiviral activity showed that the compound activity is strong at concentration 60 mg/L with survivability percentage at 34%. The result indicated that polyhydroxy isocopalane has an activity as anti-white spot syndrome activity from Litopenaeus vannamei.