Background: In patients with T2DM, the therapeutic effects of conservative treatment are quite limited, and there is a need for additional therapeutic procedures to achieve the desired satisfactory and solid effect. Low-level laser therapy (LLLT) has an anti-inflammatory effect, and is used to heal lesions. This mechanism is realized through inhibition of lipopolysaccharides (LPS), so it can be used in the treatment of periodontal disease in patients with diabetes. Objective: The aim of this study is to assess the effect of level laser therapy (LLLT) on serum IL-6 values in patients with periodontitis and T2DM. Methods: Patients at age between 35-60 years old, with chronic periodontitis (CH) where the clinical loss of attachment (CAL) was ≥4 mm therefore covering at least 50 % of affected teeth. In this study we included 80 patients, divided into two groups: 40 patients with type 2 diabetes mellitus (2TDM) treated with conservative periodontal treatment supplemented with laser therapy (LLLT), group A, and 40 patients with 2TDM, conservatively treated without LLLT. therapy i.e. group B. The laser light was applied to the gingiva in separate quadrants in 5 sessions for the next five days in a row. Blood samples were taken from all subjects at the first treatment, then in 6 weeks and 3 months after treatment, and interleukin 6 (IL-6) levels were measured. The blood samples in the test tubes remained for about 30 minutes and were then distributed in a biochemical laboratory, where they were centrifuged at 6,000 rpm for 10 minutes. The serum was separated from the test tube and transferred to the eppendorph. All serum samples were stored at -80 ° C until complete analysis and determination of IL-6, according to the standardized methodology. Results: In group A, on the first examination serum IL-6 levels varies in the interval 11.54 ± 1.11 pg / mL, after 6 weeks of therapy the values range between 11.26 ± 0.77 pg / mL, and after 3 months of therapy levels oscillate at intervals of 11.02 ± 0.67 pg / mL. In group B the findings are similar. At the first examination, the serum IL-6 values were 11.56 ± 0.81 pg / mL, after 6 weeks of therapy ranged from 11.59 ± 0.71 pg / mL, and after 3 months of therapy levels were recorded at intervals. 11.41 ± 0.78 pg /mL. The serum IL-6 value after 6 weeks of therapy in patients in group B for Z = -2.04 and p <0.05 (p = 0.04) was significantly higher than in patients in group A, while after 3 months of therapy in patients in group B for Z = -2.42 and p <0.05 (p = 0.02) is significantly higher than the value in patients in group A. Conclusion: LLLT resulted in significantly reduced serum IL-6 values in patients with periodontitis and T2DM after 6 weeks and 3 months of therapy in which conservative treatment was supplemented with LLLT.

To monitor blood triglyceride (TRG) values in patients with and without type 2 diabetes mellitus (T2DM) and chron- ic periodontitis (CP) after conservative therapy (scaling and root planning – SRP) with and without laser light ap- plication (low-level laser therapy – LLLT). A total of 120 pa- tients with type 2 diabetes mellitus (T2DM) and diagnosed chronic periodontitis (CP), where the clinical attachment loss (CAL) is ≥4 mm on at least 50% of the affected teeth, were followed. The patients were 35 – 60 years, distribut- ed in three groups A, B and C, 40 in each group. Group A consisted of patients with T2DM and CHP, where conserva- tive periodontal treatment was carried out, supplemented with laser therapy (i.e. T2DM + CP – SRP and LLLT). Group B, where T2DM and CP were diagnosed, consisted of patients treated only with SRP, without application of laser light without laser therapy (i.e. T2DM+ CP – SRP without LLLT). The group C consisted of patients diagnosed with CP, but without T2DM. In all subjects, the values of triglycerides in blood were determined with standard procedure, and then mathematically calculated. The values of TRG in blood in the group A, and the group C after 6 weeks of therapy are significantly lower than the values at the first examination. In the group B the value of TRG in blood after 6 weeks of therapy is in- significantly lower than the value at the first examination. In the group A, the value of TRG in blood after 3 months of therapy and in the group B is insignificantly lower than the value at the first examination in patients. After 3 months of therapy in the group C, the registered value is significantly lower than the value at the first examination. The blood TRG value in the group A 3 months after the therapy is insignificantly higher than the value after 6 weeks of therapy. In the group B and the group C blood TRG value is insignificantly lower than the value at six weeks after therapy. At patients with and without T2DM and CP, regardless of whether they were treated with SRP and LLLT or with SRP, without LLLT, a significant correction of blood TRG was recorded after 6 weeks, and insignificant after 3 months of treatment. SRP and LLLT treat- ment showed the highest effect in the group of patients without T2DM and CP.

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