The present study was carried out to induce cytological variations on Capsicum annuum L. (2n=24) var. Pusa Jwala by using various doses of gamma rays. Progenies were raised from the seeds of the selected cultivar treated with eight different doses of gamma rays, viz., 1, 5, 10, 15, 20, 25, 30 and 35 kR at Bhabha Atomic Research Centre, Mumbai. Cytological studies were carried out in the treated as well as in control materials. Gamma rays have shown a significant effect causing various types of chromosomal abnormalities such as univalents, multivalents, bridges, stickiness, precocious segregation, laggards and chromosome fragment.