Based on the results of pre-cycle test and observation of the fifth-grade students at Unggulan Muslimat NU Kudus Elementary School, it`s found that the average students` science literacy is low.This study attempts to discover the improvement of Unggulan Muslimat NU Kudus Elementary School fifth grade students` science literacy when apply discovery learning assisted by audio visual media. This study is a Classroom Action Research (CAR) that conducted in Unggulan Muslimat NU Kudus Elementary School, in even semester in the academic year of 2017/2018. The subjects of this research are the researcher as the teacher model and the fifth grade students that consists of 29 students. This study held for two cycles, there are two meeting in every cycle and consist of four phases: plan, action, observe, dan reflection. The independent variable of this study is discovery learning assisted by audio visual media, and the dependent variable is science literacy. Data collections of this study are science literacy test for knowledge aspect (cognitive), and observation for science literacy of attitude aspect (affective), competention aspect (psychomotor). This study is qualitative and quantitative descriptive analysis. The result of this study shows that, there is enhancement of science literacy of the fifth grade students of Unggulan Muslimat NU Kudus Elementary School in the force material, between first cycle 71,9% and improved in the second cycle 81,5% with high category. This results shows that the application of discovery learning model assisted with audio visual can improve science literacy of the fifth grade students of Unggulan Muslimat NU Kudus Elementary School.