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Objective:To analyze the genetic and clinical outcomes of blastocysts derived from 0PN oocytes after IVF/ ICSI.Methods: This retrospective observational study included patients aged 40 years or younger submitted to IVF/ICSI with their own oocytes and with blastocysts derived from 0PN oocytes between January 2015 and April 2018. The clinical outcomes of 0PN blastocyst transfers were analyzed. Genetic tests were performed on biopsied 0PN blastocysts with Next Generation Sequencing.Results: A total of 27 0PN blastocysts were transferred, yielding an implantation rate of 48.0% and an ongoing pregnancy rate of 50.0%. The transfers resulted in 13 live births (59.0% live birth rate). Genetic test results revealed that four of the 17 0PN blastocysts biopsied were 46XX; three were 46XY; and 10 were aneuploid embryos, awarding a diploid rate to 76.4% (13/17).Conclusion: Almost half of the 0PN blastocysts implanted (48.0%) and 13 healthy babies were born. More than three quarters (76.4%) of the 0PN blastocysts were diploid, thus ruling out the possibility of parthenogenetic activation. Our study indicated that the transfer of 0PN blastocysts is a safe, worthy option when the number of normal 2PN embryos is insufficient.

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This research investigates Instagram users' sentiment narrated during the lockdown period in Italy, caused by the COVID-19 pandemic. The study is based on the analysis of all the posts published on Instagram under the hashtag #andràtuttobene on May 4, May 18 and June 3, 2020. Our research carried out a view on a national, regional and provincial scale. We analyzed all the different languages and forms (i.e. captions, hashtags, emojis and images) that constitute the posts. The aim of this research is to provide a set of procedures revealing the different polarity trends for each kind of expression and to propose a single comprehensive measure.