The work on the inhalation toxicity of the ozone-air mixture was carried out in two stages. At the first stage, acute inhalation toxicity was studied and the class of toxicity was determined, at the second stage, subchronic inhalation toxicity was determined. The object of the study was an ozone-air mixture. A portable standalone ozone generation device developed by us was used as a source of the mixture. A total of 58 outbred mature white Wistar rats of both sexes were used in the study, where 18 rats participated in the first stage, and 40 rats in the second. The animals were placed in an inhalation chamber. The procedure for testing acute inhalation toxicity was carried out according to the standard method at concentrations of ozone-air mixture of 100, 500, 2500 ppm and an exposure of 4 hours. As a result, the fatal outcome was observed only in 3 groups (2500 ppm), 3 out of 6 individuals were killed, including 2 males and 1 female. In fallen animals, death occurred as a result of respiratory arrest. The surviving animals were monitored for 14 days after the test. During the treatment of animals with ozone-air mixture, a gradual inhibition of the respiratory system was observed, followed by the development of pulmonary edema and respiratory failure. Based on the data obtained, the ozone-air mixture under study was assigned a hazard class 3. In the study of subchronic inhalation toxicity of oats, concentrations of 250, 125, and 50 ppm were studied. The experiment was carried out, according to the standards for determining the subchronic inhalation toxicity of substances, for 90 days. During the experiment, body weight, feed consumption, behavioral activity were recorded, and blood hematological and biochemical parameters were determined. As a result of the study of subchronic inhalation toxicity of the ozone-air mixture, no signs of intoxication were detected in rats, and there were no death cases.
