Показана можливість швидкого налагодження масового виробництва систем штучної вентиляції легень типу «Мольфарка-Корона» з мінімально потрібними функціональними властивостями, що надасть шанс хворим на COVID-19 у найскладнішій ситуації пандемії.
The article presents the results of histological studies of topography, microstructure and functional features of the stomach lymphoid tissue in birds. The research was carried out on 12 species of wild birds belonging to 6 orders. Classical methods of histological specimens staining with hematoxylin and eosin, according to Weigert, Van Gieson, and argentum nitrate impregnation according to Kelemen were used when performing the work. It has been confirmed that the birds' stomach has glandular (proventriculus), muscular (ventriculus, gizzard) and pyloric parts, which are not equally expressed in separate species. In all parts of the stomach, lymphoid tissue is represented by local clusters, which in birds of the orders Galliiformes (P. colchicus, B. bonasia, P. crictatus); Anseriformes (B. canadensis); Passeriformes (P. pica, C. cornix); Gruiformes (G. chloropus, F. atra); Ciconiiformes (C. ciconia) and Columbiiformes (C. livia) are located in the tunica mucosa and submucosa, and in B. canadensis, F. atra, G. chloropus also in the tunica muscularis and tunica serosa. In L. lagopus and G. glandarius, lymphoid tissue has been found only in the proventriculus and its intermediate zone. Structural levels of lymphoid tissue (diffuse form, prenodules, primary and secondary lymphoid nodules) are unequally expressed in the birds' stomach. In B. bonasia, B. canadensis, P. pica, C. cornix, C. ciconia, C. livia, only a diffuse form appears in all its parts. This form of lymphoid tissue is registered in the ventriculus of other birds species and proventriculus in L. lagopus and G. glandarius. All levels of the lymphoid tissue structural organization are detected in the proventriculus in P. crictatus, G. chloropus and in the proventriculus and pyloric part of the stomach in P. colchicus and F. atra. The content of lymphoid tissue is different in parts of the birds' stomach. Most of all it is contained in the intermediate zone of the proventriculus and the pyloric part of the stomach. Diffuse form is the most common among the levels of lymphoid tissue structural organization. The research results make it possible to more fully assess the morphofunctional status of wild birds and, accordingly, to find out the state of their immune system
The studies were conducted on 2-3-months-old males of C57BL/6 mice weighing 20-24 g. Obtaining and cultivating of adipose-derived mesenchimal stem cells (AD MSCs) were carried out in a sterile laminar box with compliance of conditions of asepsis and antiseptics. AD MSCs of the 2, 4, 7 and 12 passages were analyzed. Morphometric analysis was performed using a light microscopy. Morphometric parameters such as cell and nucleus area or nuclear-cytoplasmic ratio (NCR) were calculated using the Axiovision light microscope (Carl Zeiss, Germany) and ImageJ 1.45 software. Trypan blue dye used for investigation of the viability of MSC. The morphological characteristics of mesenchymal stem cells from adipose tissue during the process of cultivation changes: at the first passages of cultivation, the cells are spindle-shaped with two, at least three, long long cytoplasmic processes, located bipolar. Near the nucleus the Golgi complex is clearly visible -a sign of active cells. At later passages cells have a small cytoplasmic processes and the bipolar arrangement of processes changes by stellar arrangement. Golgi complex is also clearly visualized. The indicator of the nuclear-cytoplasmic ratio in MSC from adipose tissue is significantly reduced at 7 passage to 0.2189 ± 0.0122 (P < 0.01), and at 12 passage to 0.1111 ± 0.0086 (P < 0.001) compared to the 2 passage. The coefficient of proliferation of MSC from adipose tissue is significantly reduced at 12th passage. The viability of mesenchymal stem cells from adipose tissue with an increasing of a number of passages significantly reduces and at the 12th passage of cultivation reaches 84,67 ± 1,36 * (P < 0.05). The content of apoptotic cells that exhibited sensitivity to serum-free significantly increased at 7 and 12 passages and was respectively 21.33 ± 1.36 (P < 0.05) and 23.67 ± 0.97% (P < 0.05).
Rabbits are an important livestock animal species, which are used for their meat and fur. Nowadays they are also becoming more popular as pets. Furthermore, rabbits are commonly used in research, inter alia in immunological studies and for studying pathogenesis of human and animal diseases. The lymphoid tissue is abundant in the rabbit intestine and a lot of it is concentrated in Peyer's patches, the majority of which is located in the jejunum. Understanding of the rabbit Peyer's patches functions is essential for the prevention and treatment of their diseases. In order to enhance it, accurate knowledge of its lymphocyte population composition is needed. At present, the cellular composition of the rabbit gut-associated lymphoid tissue remains insufficiently studied. Therefore, the aim of our study was to establish the content and localization of lymphoid populations in the domestic rabbit jejunal Peyer’s patches. We performed immunohistochemical studies using monoclonal antibodies CD3, CD10 and CD20 (DAKO, Denmark) to identify the distribution of lymphoid populations in jejunal Peyer's patches of a mature domestic rabbit. The results, obtained in our study, complement the existing data and determine the main lymphoid populations in the rabbit jejunal Peyer's patches. The presented data are fundamental for further studies of the rabbit gut-associated lymphoid tissue. We found that lymphoid nodules of jejunal Peyer's patches contains a predominant CD20+ cell population (B cells). It also contains many CD10+ cells (precursors of T cells and B cells), which are mostly concentrated in the germinal centers of lymphoid nodules. In the diffuse form of the lymphoid tissue the vast majority of cells are CD3+ cells (T cells)
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