One of promising directions in oncology is the development of methods for treatment of malignant tumors with account of mechanisms of their genesis and focused on correction of definite pathogenesis components, while being nontoxic for other organs and tissues. Investigated in this work was the possibility to use the phenomenon of surface plasmon resonance (SPR) for analysis of blood inherent to patients with glioma of different degrees of malignancy by studying the level of aggregation of blood cells. For this aim, we ascertained correlation between SPR characteristics and the condition of patient as well as choice of the drug efficient concentration. The works were performed being based on positive results preliminary obtained in experiments with lab rats, which were carried out in cooperation with the State Institution "A.P. Romodanov Institute of Neurosurgery, NAMS of Ukraine", and V. Lashkaryov Institute of Semiconductor Physics, NAS of Ukraine by using the device "Plasmon". It has been experimentally shown that, when using the chosen drug by treated patients, the signs of toxical action of verapamil hydrochloride are absent, and the life span is 10 months longer as compared with the group of patients that are not treated with this drug in low concentrations during the postoperative period.

Mechanism of tumor progression in malignant gliomas and other tumors of the body were studied to identify the main pathogenetic link. Indicators of the charge of cell membranes, including the membranes of blood cells, can be considered in vein. These indicators are universal for any type of tumors, and not tissue-specific. Indicators of many significant processes in the body depend on the level of cellular charge, which emphasizes its dominant role. Interaction of reparative processes of inflammatory genesis with regenerative processes carried out by blood stem cell in relevant studies and in this study by mesenchymal blood stem cells. The interaction is under the control of the epidermalmesenchymal transition depends on a large extent on the cellular charge of blood cell membranes. The paper presents the correlation features between the pools of cells of inflammatory and regenerative origin (leukocytes, lymphocytes, granulocytes, and monocytes), the charges of their membranes in gliomas of III grade of malignancy and meningiomas of I grade of malignancy studied in vitro. Membrane charges were determined indirectly, through the level of aggregation of blood cells, using the surface plasmon resonance method where the aggregation expressed in arbitrary SPR units. To detect a latent correlation, low concentrations of verapamil hydrochloride (10,000-fold dilution) were added to the blood samples before determining the level of cell aggregation and the samples were exposed to low-level laser radiation with an oscillation frequency of 1.2 Hz. Results indicate the great importance of maintaining a normal, rather than reduced, level of cell membrane charge.