This study highlights a persistent transmission network of EV-A71, with specific Asian countries seeding other countries in the region and beyond, emphasizing the need for improved EV-A71 surveillance and detailed genetic and antigenic characterization.

This paper summarizes different approaches to the meaning extension of the English preposition over and proposes a multimodal approach comprising three spatial image theories and one mental space theory in reference to an image-based view. It is concluded that the author’s proposal is a combination of Deane’s 2005 multimodal spatial representations and  2017 Kövecses’s model, in which there is an emphasis that treating the spatial configurations of a spatial marker requires different frames and when the marker denotes a non-spatial sense, there exists an activation of a metaphor layered from its frame in certain context with a specific communicative purpose to the domain of which the frame is a part and finally the activation will reach the image schema that supports the frame.

This paper analyses the transference from spatial to non-spatial meanings of below as a preposition, an adverb, and a particle within the 2017 Corpus of Contemporary American English, genre: fiction. In light of the Multimodal Image Theory and Extended Conceptual Metaphor Theory, the data analysis shows that the prototypical meaning of below has two variants in its Visual space, and two non-spatial meanings namely Less and Inferior. The mechanism of meaning transference is mappings in which salient aspects of absence of contact, force, and occlusion between the Trajector (TR) and Landmark (LM) are retained in construing the non-spatial scenes associated with the word.

Chloroplasts and mitochondria are organelles that have their own genome in a cell. The chloroplast genome provides information on the evolutionary relationship and species identification, valuable markers for transgenic plants, and cloning plants, etc. The application of Next Generation Sequencing has improved the chloroplast genome sequencing. However, the assembly process of chloroplast genome is quite complicated due to the need of different complex bioinformatics tools, high configuration computer and laborous. Here we configured the process of assembling the chloroplast genome of Paphiopedilum delenatii. The assembled chloroplast genome was 160,955 bp in length, including a large and a small single copy region (LSC, SSC) separated by a pair of inverted repeats (IR). Total genes were 130 genes, GC content is 35.6%. Genome data was mapped and registered in GenBank under accession number MK463585. The optimal parameters for genome assembling were recommended. This study not only provided information for conservation of the Vietnam endemic Paphiopedilum delenatii species but also supported the genome assemble researches which could be applied on other subjects.