The genus Goniothalamus comprises about 160 species of shrubs and trees growing in tropical and subtropical Asia. 1) Many of these species are used for timber, as fiber sources, as mosquito repellant, and most interestingly in folk medicine in several countries. 2,3) Phytochemical studies of Goniothalamus species have led to the isolation and characterization of a large number of styryl-lactones. These can be classified into several main subtypes: styryl-pyrones, furano-pyrones, furano-furones, and pyrano-pyrones. 4) Styryl-lactones possess significant cytotoxic activity against several human tumor cell lines such as A-549 (lung carcinoma), HT-29 (colon adenocarcinoma), MCF-7 (breast carcinoma), RPMI (melanoma), U251 (brain carcinoma), 3,5) and to induce apoptosis in HL-60 leukemic cell. 6) Some of styryl-lactones have been synthesized via total synthesis. 3,7-10) Goniopypyrone, the first pyrano-pyrone compound, was first isolated in 1990 from G. giganteus. 11,12) It was high bioactive, showing ED 50 values of ca. 0.67 mg/ml in the cytotoxicity of human tumor cell lines (A-549, MCF-7, HT-29), high toxicity to the brine shrimp (BS), and significant inhibition of the formation of crown gall tumors on potato discs (PD). Typically, up to date, pyrano-pyrone compounds are found in some Goniothalamus species.The formation of bone involves a complex series of events, including the proliferation and differentiation of osteoprogenitor cells. Eventually, this results in the formation of a mineralized extracellular matrix. Most effective osteoporosis therapies reduce bone loss but do not restore lost bone mass and strength. Therefore, it is desirable to discover bone-building (anabolic) agents that stimulate new bone formation and correct the imbalance of microarchitecture characteristic of established osteoporosis. 13) As new bone formation is primarily a function of the osteoblasts, agents that regulate bone formation act either by increasing the proliferation of cells in the osteoblastic lineage or inducing osteoblast differentiation. 13,14) Early intervention is now possible with the help of some effective medications, which may reduce the risk of first and recurrent fractures.In our investigation of phytochemistry of the leaves of G. tamirensis, 9-deoxygoniopypyrone (2) and a new pyranopyrone, (ϩ)-8-epi-9-deoxygoniopypyrone (1), were isolated and identified using spectral methods. To investigate whether these compounds could stimulate the function of osteoblasts, their effects on cell growth, collagen content, alkaline phosphatase (ALP) activity, and calcium deposition were assessed in the pre-osteoblastic target cell line, MC3T3-E1, which is a well-characterized in vitro model of osteoblast differentiation. 15) Results and DiscussionCompound 1 Science and Technology; 18 Hoang Quoc Viet St., Caugiay, Hanoi, Vietnam: and c Faculty of Chemistry, Hanoi University of Science; 19 Le Thanh Tong St., Hoankiem, Hanoi, Vietnam. Received November 24, 2009; accepted January 8, 2010; published online January 19, 2010 The n...

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Sponges (Phylum Porifera) are ancient sedentary and filter-feeding animals which harbour very diverse and abundant associated microbial community in their tissues with density up to 40-50% of sponge tissue volume. In this study, the diversity of associated microorganisms with two marine sponges Haliclona oculata and Amphius huxleyi collected at the Lang Co bay of Vietnam was assessed by analysis of hypervariable V3 and V4 regions of the 16S rRNA gene using Illumina MiSeq system. The taxonomic diversity of sponge-associated microorganisms was classified to different taxonomic levels (kingdom, phylum, class, order, family, and genus). Based on Bayesian classification method and reference sequences derived from Greengenes database, the associated microorganisms in studied sponges were assigned to 17 phyla (H. oculata) and 13 phyla (A. huxleyi). Many microbial taxa were detected in two sponge species, however, they were distinctive by the abundance. Proteobacteria was the most dominant phylum in both sponge species, and all of 4 classes Epsilonproteobacteria, Gammaproteobacteria, Alphaproteobacteria, and Deltaproteobacteria were found in H. oculata and A. huxleyi.

Introduction: Proteases are enzymes that catalyze the hydrolysis of peptide bonds and play an important role in almost all biological processes. However, excessive protein proteolysis can be implicated in several diseases, such as cancer, as well as cardiovascular, inflammatory, neurodegenerative, bacterial, viral and parasitic diseases. In these cases, protease inhibitors can be used as one of versatile tools for regulating proteolytic activity of target proteases as well as therapeutic applications. In this study, we expressed and characterized a new serine protease inhibitory protein (PI-QT) from the metagenome of sponge-associated microorganisms in Escherichia coli. Methods: The gene PI-QT encoding for a new serine protease inhibitory protein was expressed in E. coli BL21(DE3). In addition, the expressed protein was purified and characterized. Results: Optimization of expression of the recombinant protein PI-QT in E. coli showed that suitable conditions for expression of the protein were pre-induction cell density (OD600) of 0.6 - 0.7, IPTG concentration of 1 mM and temperature of 25oC. The protease inhibitory protein was also purified and identified by mass spectrometry LC-MS/MS. The recombinant protein showed inhibitory activity against trypsin anda-chymotrypsin with activity values of 97526 U/mg and 41714 U/mg, respectively. Maximum activity of the protease inhibitory protein was obtained at pH 7 and temperature 20-35oC. The inhibitor was stable over pH 4-9 and up to temperature 50oC. Addition of Zn2+, Mg2+ and Ca2+ enhanced inhibitory activity, whereas other metal ions, surfactants and oxidants reduced inhibitory activity of the protease inhibitor. Conclusion: The recombinant protein PI-QT is a potential protease inhibitor for therapeutic applications.

Mục tiêu: Đánh giá chất lượng sống (CLS) liên quan đến sức khỏe ở trẻ mắc Basedow. Đối tượng: 80 trẻ mắc Basedow từ 8 đến 18 tuổi đã được chẩn đoán, điều trị và theo dõi điều trị tại bệnh viện Nội tiết Trung ương từ tháng 7/2020 đến tháng 6/2021, được đánh giá CLS liên quan đến sức khỏe bằng thang đo PedsQLTM 4.0. Phương pháp: Nghiên cứu mô tả. Kết quả: Điểm CLS liên quan đến sức khỏe do trẻ mắc Basedow (8-18 tuổi) tự báo cáo trong các lĩnh vực thể chất, cảm xúc, quan hệ xã hội, học tập và CLS tổng quát lần lượt là: 80,16 ± 11,61; 68,63 ± 10,99; 83,94 ± 7,06; 73,50 ± 9,12; 77,02 ± 8,44. Điểm CLS do bố/mẹ trẻ mắc Basedow báo cáo tương ứng lần lượt là: 79,65 ± 7,80; 71,75 ± 10,47; 83,44 ± 6,68; 75,19 ± 6,29; 77,79 ± 5,94. Điểm CLS do trẻ Basedow báo cáo thấp nhất ở lĩnh vực cảm xúc. Có sự khác biệt về điểm CLS giữa trẻ và bố/mẹ trẻ báo cáo ở lĩnh vực cảm xúc và học tập, tuy nhiên chưa có sự khác biệt giữa điểm CLS tổng quát do trẻ báo cáo và bố/mẹ trẻ báo cáo. Có sự suy giảm về CLS do trẻ Basedow báo cáo ở nhóm tuổi 13-18 tuổi so với nhóm tuổi 8-12 tuổi ở phần lớn các lĩnh vực. Điểm CLS do trẻ mắc Basedow báo cáo ở cả hai nhóm tuổi thấp hơn đáng kể so với điểm CLS do trẻ khỏe mạnh cùng độ tuổi báo cáo, đặc biệt ở lĩnh vực thể chất, cảm xúc, học tập và CLS tổng quát. Kết luận: Chất lượng sống liên quan đến sức khỏe ở trẻ mắc Basedow bị suy giảm so với trẻ khỏe mạnh cùng độ tuổi ở hầu hết các lĩnh vực, đặc biệt trong lĩnh vực thể chất, cảm xúc và học tập.