The article presents the materials of the study on bull cross of the Bredy meat type simmentals, which include the genotyping of animals for the CAST and CAPN1 genes with the identification of frequencies of genotype and alleles, as well as testing the production of these animals expressed in the evaluation of aged within 8 days of meat for the indicators tenderness, juiciness, organoleptic assessment of taste. The results of the studies did not confirm, with certainty, the influence of the CAST gene polymorphism on the beef tenderness indicators, as well as no associations of this gene with the studied juiciness and meat taste indicators, while the carriers of the homozygous CC genotype CAPN1 exceeded other genotypes of the sample in tenderness, juiciness and taste of beef. And indicators of bull meat with a combination of TT* genes on CAST and CC* on CAPN1 had superiority over the average indicators of the total sample (P <0.001), as well as over the group of animals with the most preferred CC genotype for the CAPN1 gene by 1.22 points or 15.4% (P <0.01) by tenderness, by 1.44 points or 18.2% (P <0.05) by juiciness, by 1.56 points or 19.2% (P <0.001) by taste. Studies have confirmed the effect of the CAPN1 gene CC homozygotes polymorphic state itself, as well as the CAST and CAPN1 genes on beef tenderness in the part where the combination of TT genotypes in the CAST gene and CC in the CAPN1 gene gives a positive effect on beef tenderness, probably associated with activation the activity of μ-calpain in connection with the weakening of the effect of calpastatin as an inhibitor on μ-calpain, as a result of which the enzymatic effect on myofibrillary proteins acquires a different intensity, and possibly a different manifestation. What was the reason for the increase in sensory sensitivity to taste of prepared samples, tenderness and juiciness of meat.

A comparative study of the biological characteristics of the heifers Bredy meat type of two populations located in Russia and Kazakhstan was carried out to determine the state of genes associated with the meat quality in the genotypeCAPN1, GDF5, TG5, BGL. In genotyping, we used the real-time PCR method using oligonucleotide primers (Gen Bank), for each primer individually in the corresponding sequences. Studies for improve breeding by innovative methods revealed the frequencies of genotypes and alleles for marker genes CAPN1, TG5, BGL and GDF5, which are related to meat quality indicators, a certain displacement in the genotypes of the compared populations of the Bredy meat type of Kazakhstan and Russia according to the CAPN1 gene (χ2 = 8.52) and the TG5 gene (χ2 = 4.73). The genotyping result of animals from LLC “Breeding plant Bredy” were analyzed from the point of view of attributing carriers of different genotypes to the offspring of bulls-producers Fakir, Kust and Chizhik, in order to determine the producer giving offspring with predictably high nutritional and biotechnological properties of beef. A comparison of the carriers of the identified genotypes of the studied genes by the characteristics used in the evaluation of young animals by their own productivity did not give significant results, however, showed trends by which scientific research can be directed. An assessment of the bulls-producers of the successors of the different line of meat simmentals on the quality of heifers – daughters showed that the largest comprehensive index was set for the bull-producer Bush 39046 – 102.7, which was recognized as an improver. The assignment of all, without exception, heifers of different groups, evaluated bulls-producers, to the highest class elite-record, indicates the value of all the studied genotypes.

No abstract

Представлены результаты исследования по выявлению хозяйственно-биологических особенностей и селекционно-генетических параметров селекционируемых признаков коров стада абердин-ангусской породы австралийской репродукции. По результатам исследования выявлено, что генетический потенциал продуктивности максимально выражен в возрасте пяти лет (у полновозрастных коров) и перманентно сохраняется до девятилетнего возраста. Рост основных селекционируемых признаков с двух до пяти лет составил по живой массе 15,1%, по молочности – 5,1%. С увеличением возраста маток отмечен стабильный рост показателя «выход деловых телят» на 2,3%. Селекционный дифференциал за три поколения у коров в трёхлетнем возрасте по живой массе составил 23 кг и по молочности – 7,4 кг, коэффициент наследуемости составил 0,42 по живой массе и 0,36 – по молочности коров, эффект селекции по живой массе за поколение составил 9,7 кг, за год – 1,9 кг, по молочности – 2,7 кг и 0,5 кг соответственно. У генотипированных коров по схеме мать-дочь эффект селекции за поколение был выше у гетерозиготных генотипов дочерей, рождённых в Курганской области. Знание и рациональное использование традиционных и новых по ДНК маркерных методов селекции на производстве позволит выявить, а также реализовать генетический резерв животных и управлять селекционным процессом при совершенствовании племенного стада абердин-ангусской породы. The results of research on detecting of commercial-biological features and selection-genetic parameters of cows’ features under selection of the Australian reproduction Aberdeen-Angus breed herd of are presented. According to the results of the research it was revealed that the genetic potential of productivity is maximally expressed at the age of 5 years (in mature cowbanes) and persists permanently until the age of nine. The growth of the main features under selection from two to five years amounted to 15.1% by live weight, 5.1% by milking capacity. With an increase in the age of the dams a stable increase in the "calf crop percent" by 2.3% was noted. The selection differential for three generations in cows at the age of three years in living weight was 23 kg and in milking capacity – 7.4 kg, the inheritance coefficient was 0.42 in living weight and 0.36 - in milking capacity of cows, the effect of selection by live weight per generation was 9.7 kg, for the year – 1.9 kg, in milking capacity – 2.7 kg and 0.5 kg, respectively. In genotyped cows according to the dam-daughter scheme genetic progress through selection per generation was higher in heterozygous genotypes of daughters born in the Kurgan region. Knowledge and rational use of traditional and new DNA marker methods of selection at the place of production will allow identifying, as well as unlocking the genetic reserve of animals and managing the selection process while improving the breeding herd of the Aberdeen-Angus breed.

The problem of developing techniques and tools for integrated adverse effects on soil properties at the current level remains a challenge. The aim of the research was to identify the influence of fertilizing-reclaiming mixtures, prepared on the basis of local raw materials, the change of the ecological state of the black earth degraded. The field experience. Repeated three times. Bookmark and conducting experience, as well as analyzing soil samples was performed according to standard techniques. Reclamation of the black earth degraded fertilizing-reclaiming mixtures contributed to the normalization of the ecological status of these soils, namely: reduction of salonauto to 1-2 % of sodium from the sum of the soil adsorbing complex, to eliminate alkalinity and decompaction of the soil to 1,14 and 1,7 t/m 3. Fertilizing-reclaiming mixture, created on the basis of local raw materials, at its meliorative and environmental impact on ordinary Chernozem not inferior degraded compost made of poultry manure and phosphogypsum, but are more slow-acting means in comparison with them.