Busulphan and Endoxan inhibit the normal course of ovarian follicular growth. Older secondary as well as an antral follicles perish within a very short time. Growing follicles, which at the beginning of the experiment exhibited only one layer, remained intact and single-layered during the entire duration of the experiment. The oocytes, however, continue growing, and the cytoplasmic structures, which are characteristic of older growing follicles, develop in them as well as in the follicle cells. Even a theca formation develops. In some of the "growing" follicles which have remained single-layered, after 10 days of Busulphan administration, some liquor folliculi is produced and accumulates in a fissure-shaped "antrum" between the zona pellucida and the follicular epithelium.

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The specific conditions of osmium fixation have been studied on gelatin gels and bovine pancreas. Protein (gelatin)-bound osmium can be quantitated by a modification of the Majumdar and Sen Gupta test, free and tissue (pancreas)-bound osmium by a modified Bahr test. Using these two methods, the depth of OsO4 penetration after increasing the times of fixation, the osmium concentration gradients and the total concentration of free and bound osmium at given spots within gelatin and tissue blocks have been determined. Warburg type experiments on suspended yeast cells and leukocytes demonstrate that 1% OsO4 stops cell respiration completely only after 40-min incubation. Formalin, 1%, is a more effective respiratory inhibitor.

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