Lobster (Panulirus homarus) or spiny lobster is one of the export commodities of the Indonesian fisheries subsector and an important component for shrimp fisheries in Indonesia. This commodity needs to be further developed because of its high trade value and potential. Lobster enlargement cultivation in Indonesia began in early 2000, with the discovery of puerulus attachments in the waters of the bay of Lombok. However, in this lobster cultivation business failure often occurs due to the high mortality caused by infectious diseases which are generally caused by bacterial, parasitic, fungal, and viral infections. The bacteria that infect lobsters can reduce the expected production target. However, bacteria in lobster culture could have beneficial or harmful effects, depending on the prevailing conditions. This study aims to identify bacteria in spiny lobsters (Panulirus homarus). Bacterial isolation came from organs in spiny lobsters, that are hepatopancreas and hemolymph. Bacteria isolates were identified through their colony morphology and biochemical tests. Characterization on the Thiosulfate Citrate Bile Salt Sucrose medium showed that lobsters were infected with Vibrio. Based on Bergey's Manual of Determinative Bacteriology and Nial, the results showed that the Hepatopancreas in first lobsters were infected with Vibrio algynolyticus that mostly in the Hepatopancreas. The second sample was found to be infected with Vibrio damsela that mostly in the Hepatopancreas. However, no bacteria were found in spiny lobster hemolymph.

Spiny lobster (Panulirus homarus) is one of the export commodities of the Indonesian fisheries subsector and an important component for shrimp fisheries in Indonesia. In the development of lobster cultivation, there are several obstacles, the presence of vibriosis infection caused by the pathogenic Vibrio bacteria. This study aimed to identify Vibrio spp. bacteria in spiny lobsters (P. homarus) reared in the marine cultivation ponds, Bengkulu, Indonesia. Clinical symptoms of lobsters infected with vibriosis are red spots on the uropod, pleopod, and abdominal parts. Bacterial isolation was conducted by isolated some internal organs in spiny lobsters, that are, gills, stomachs, haemolymph, and hepatopancreas. The result showed there are 5 isolates of Vibrio bacteria that coded by IN3, ST2, HA1, HP2, and HP3. These bacteria isolates were identified through their colony morphology and biochemical tests. Characterization on the Thiosulfate Citrate Bile Salt Sucrose (TCBS) medium showed that lobsters were infected with Vibrio species. Based on Bergey’s Manual of Determinative Bacteriology, Austin and Austin, the identification results showed that HA1 isolate was identified as Vibrio algynolyticus, IN3 isolate was identified as V. anguillarum, ST2 was identified as V. ordalii, HP2 in first lobster was identified as with V. algynolyticus that mostly in the hepatopancreas, and HP3 was identified as V. splendidus