A method was developed for producing several hundred mCi of 13NH 3 with subsequent enzymatic synthesis of large amounts ot' 13N-labeled glutamine and glutamic acid. Dynamic measurements and quantitative whole body scans demonstrated greater glutamine uptake in the liver region of mongrel dogs than uptake of glutamic acid or NH 3, although all concentrated in the liver to a significant degree. Myocardial uptake of glutamine and glutamic acid was low, while NH 3 was incorporated into the heart, brain, bladder, and kidneys at a greater rate than the amino acids.

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