No abstract

METHODS There are three stages in the procedure: taking blood into PHA, centrifuging the blood and incubating the cells that remain suspended. These stages will be described separately. Each maneuvre was carried out using standard sterile precautions.Blood Taking.-The subjects, healthy men aged 20-38 years, had fasted for at least 3 hr. to avoid excessive lipamia. Approximately 300 ml. of blood was withdrawn from an antecubital vein directly into a bottle containing 30 mg. dry heparin. Care was taken to avoid frothing. Aliquots (20 ml.) of this blood were transferred to McCartney bottles. These bottles had siliconed rubber washers in their caps and each contained 04 ml. of a solution of PHA made up as follows: 5 ml. distilled water was added to each vial of PHA (phytohaomagglutinin P. Difco *) to dissolve the dry

SUMMARYA young woman with familial polyposis of the colon, multiple sebaceous cysts and osteomata (Gardner's syndrome) presented with Cushing's disease associated with carcinoma of the adrenal gland. This appears to be the first recorded case of Gardner's syndrome with adrenal carcinoma, and the first case wherein a metastasizing tumour other than carcinoma of the colon has been fully documented.