Objective: The study evaluated the anti-tubercular activity of crude extracts and active compounds which was isolated, purification and characterization from noni (Morinda citrifolia Linn) against Mycobacterium tuberculosis strains H37Rv. Methods:The crude extract of the noni fruits was macerated using ethanol (96%). Ethanol crude extracts were qualitatively screened to identify of the flavonoid, scopoletin, antraquinon and alkaloids using phytochemical fractionation by harborn method. The anti-tubercular activity of noni fruit and its combination were determined by the minimum inhibitory concentration (MIC) of the bacterial growth at various doses 10 mg/ml, 20 mg/ml, 30 mg/ml, and 40 mg/ml using agar well diffusion method. Statistical analysis was performed by analysis of variance. Results:Crude extract and active compounds of noni fruit such as flavonoid, scopoletin, antraquinon and alkaloids significantly work as antitubercular against Mycobacterium tuberculosis strain H37Rv at various doses (p value=0,000). Crude extract (59.00±60.513), alkaloids (64.83±49.356), anthraquinones (69.50±50.396), and flavonoid (72.92±58.728) showed the highest anti-tubercular activity in inhibiting the growth of Mycobacterium tuberculosis strain H37Rv compared to scopoletin (95.92±33.280) and the negative control (189.25±33.280). The minimum inhibitory concentration was found at a dose of 40 mg/ml. Conclusion:Crude extract of noni fruit was the most active anti-tubercular against Mycobacterium tuberculosis strains H37Rv. One can assume that noni fruit can be fruther researched to be used as adjuvant therapy for anti-tuberculosis drugs.