The role of HDL in reverse cholesterol transport is well known but other atheroprotective mechanism, e.g. antioxidant capacities of HDL is not clear. The aim of this study is to determine the effects of different doses of HDL on antioxidant activities assay. HDL was isolated from plasma by single step doses of HDL were measured by ferric thiocyanate (FTC) (DPPH) and dichlorofluorescein (DCF) lag time and lower reaction rate in a dose HDL had inhibitory effects on radical oxygen free radical scavenging activities. HDL is a potent antioxidant in terms of inhibition of lipid peroxidation, ROS production and LDL oxidation. These may to some extent add to the antiatherogenic beyond reverse

This study was conducted to investigate the effects of secretion and gene expression of adhesion molecule-1 (VCAM-1) interleukin-6 (IL-6) and tumor necrosis factor alpha ( cells (HUVEC). Lipoploysacharides concentrations of HDL (20-120 mg/dL) for 16 hours measured by ELISA. Total RNA was extracted and measured by Taqman real-time polymerase chain reaction assay. to investigate the effects of high-density lipoprotein ( secretion and gene expression of intercellular adhesion molecules-1 (ICAM-1), vascular cell 1), endothelial-leukocyte adhesion molecule-1 (E tumor necrosis factor alpha (TNF-α) by human vascular endothelial ipoploysacharides-stimulated HUVEC was incubated with different 120 mg/dL) for 16 hours. The levels of all biomarkers were . Total RNA was extracted and all bioamarkers' mRNA levels were time polymerase chain reaction assay. density lipoprotein (HDL) on 1), vascular cell 1 (E-selectin), by human vascular endothelial HUVEC was incubated with different biomarkers were mRNA levels were Research Article Special Issue S. Muid et al. J Fundam Appl Sci. 2017, 9(6S), 316-330 317 Different HDL concentrations reduced the secretion and expressonof all biomarkers except VCAM-1, which may contribute to the prevention and regression of atherosclerosis.