Two natural insect enemies, Chrysoperla carnea (Stephens) and Trichogramma evanescens (Westwood) and one insecticide, Profenofos, were assessed against the cotton leafworm, Spodoptera littoralis (Boisd.) under laboratory conditions. The first, second and third larval instars of C. carnea consumed 35.53±7.82, 35.82± 3.21 and 701.75± 135.6 eggs of S. littoralis, respectively. The second and third instar larvae consumed 166±39.1 and 729.91± 120.50 larvae of S. littoralis. The feeding capacity of second larval instar of C. carnea on eggs and larvae of S. littoralis differed significantly. T. evanescens parasitism was 71.05%, also the longevity of females and females percentage were 3.19 days and 39.00%, respectively. Oleander leaves, Nerium oleander infested with S. littoralis eggs were treated by dipping in 5 concentrations of profenofos, and LC50, LC90 were 39.19 and 639.681 ppm after 4 days from treatment. Moreover,The results showed that eggs mortality percentage were 43.19, 67.19, 75.20, 85.20 and 85.61% as a result of profenofos application with different concentrations 37.5, 75, 150, 300 and 600 ppm, respectively, compared with those of untreated eggs, (16.66%). This study reveals the importance of mass-rearing of biological control programs of C. carnea and T. evanescens to be successfully used in control of S. littoralis, and thus, the use of insecticides could be minimized or avoided.