Objective: This study was carried out to evaluate and compare the effect of PRF, β-TCP, and combination of these materials as periapical bone defect filling materials on the resulted bone density, cavity height, and cavity width by using cone beams computed tomography (CBCT) imaging. Patients and methods: This study was conducted on twenty-four patients with critical periapical bone defect about 2.5 cm in dimension in anterior region of maxilla resulting from periapical lesion, The patients were divided equally into three groups: Group A: the defect was filled with PRF alone. Group B: the defect was filled with β-TCP alone. Group C: the defect was filled with combination of PRF/ β-TCP. Results: The results of this study revealed that significant increase in bone density, as well as significant decrease in bony defect width and height in the three different studied groups after six months when compared to the baseline. Conclusion: The treatment of the periapical bone defects with different bone graft materials helps to increase the bone density and reduce the bone defect dimensions. The adjuvant uses of combination of PRF/ β-TCP in the treatment of preapical bone defect may contribute to more favorable and predictable bone formation at the grafted site.