Open Access Research Articlesuch as smoking habit, have a role in varying this prevalence [2]. To control such widespread infection, a recent quadruple treatment regimen combining proton pump inhibitor, clarithromycin, amoxicillin and nitroimidazole has been effectively used [5]. H. pylori is present on the gastric mucosa of less than 20% of persons under age 30 but increases in prevalence to 40-60% of persons age 60, including persons who are asymptomatic [5].In developing countries, the prevalence of infection may be 80% or higher in adults. Person to person transmission of H. pylori is likely because intrafamilial clustering of infection occurs [6].In Sudan, information about the prevalence of H. pylori infection is very patchy. and there is only one study which showed high prevalence (80%) of H. pylori infection among patients with symptoms of gastritis, 56% with duodenal ulcer, while 60% with duodenitis and 16% apparently look normal [7].Colonization of human gastric mucosa by H. pylori stimulates specific cellular and humoral immune responses to bacterium [8]. Measurement of specific antibodies in serum has been used as non-invasive method by which H. pylori infection is detected [9]. Specific immunoglobulin M (IgM) antibodies can be detected shortly after has infection occurred, but IgA and IgG antibodies titers indicate chronic infection [9].Early and prompt accurate diagnosis of H. pylori infection is crucially needed to identify any gastric diseases, limit further pathological complications and give rapid treatment to manage infected cases. The most frequent used diagnostic laboratory techniques are culture, stained smears, urease test, PCR assay, Western immunoblotting and histopathological examination of biopsy specimens [10,11]. However, these methods necessitate obtained gastric biopsy materials by an endoscopic invasive procedure [12]. Besides, cultural methods are time-consuming and show low sensitivity [11]. For this reason, serological assays such as Enzyme-linked immunoabsorbent assay (ELISA) have been utilized as non-invasive substitutes for diagnosis of infection [13]. AbstractA total of 90 patients with gastrointestinal symptoms, and who attended Khartoum Teaching Hospital during the period from April to October 2012, were enrolled in this study. Out of them 35 (38.9%) were males and 55 (61.1%) were females, their age ranges from 15-60 years with mean (33.2). The aim of this study was to detect frequency of Helicobacter pylori IgM, IgG antibodies, and to determine the relationship between the presence of antibodies and certain factors such as (sex, age and genetic susceptibility).90 serum specimens were collected from patients, and analyzed by ELISA technique. The results showed that 19 (21.1%), 57 (63.3%) were positive for IgM antibody and IgG respectively, while 15 (16.7%) were positive for both. Females were more affected than males, high frequency of positive results was observed among 15-30 age groups. Statistical analysis showed that there was insignificant correlation between age, ge...