bEquine sarcoids are locally aggressive fibroblastic neoplasms considered to be the most common skin tumors of horses worldwide. Bovine papillomavirus types 1 and 2 have typically been associated with sarcoids in equids. Investigations aiming to identify papillomavirus strains, aside from bovine papillomaviruses 1 and 2, which might be associated with sarcoid lesions, have been lacking. The aim of this article is to report the identification of a third bovine papillomavirus type, bovine papillomavirus 13, associated with equine sarcoids. Six sarcoid lesions were collected from diverse anatomical sites on two horses from southern Brazil. To detect a broad spectrum of papillomavirus strains, eight degenerate primer pairs designed to detect conserved regions on the L1 and E1 genes were tested on the DNA samples. Direct sequencing was then performed on the obtained amplicons, and sequence identities were compared with sequences from all bovine papillomavirus types. The FAP59/FAP64, MY09/MY11, and AR-E1F2/AR-E1R4 sequences generated from the sarcoids were shown to present 99 to 100% identity with bovine papillomavirus 13, a new bovine papillomavirus type previously described in cattle. The results from this study suggest that there is a need to identify bovine papillomavirus type 13 and other papillomavirus strains that might be associated with sarcoids in diverse geographical areas; such investigations might establish the frequency of occurrence of this viral type in these common tumors of equids.

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The study of the prevalence of anti-leptospiral antibodies in pet dogs in urban areas and the variables associated with the risk of infection can aid in the identification of the serovars present in the region and indicated the reservoirs involved in this important zoonosis. The aim of the present study was to determine the prevalence of anti-leptospiral antibodies in 653 dogs from 369 households distributed throughout the urban area of the municipality of Jataizinho, Paraná (PR), and to investigate the variables associated with the risk of leptospiral infection in these animals. Serum samples were subjected to microscopic agglutination test (MAT) against 23 serovars of pathogenic leptospires. Among the 132 (20.21%) reactive samples, 15 (11.36%) were reactive for more than one serovar, and the most likely serovar could not be determined. Among the 117 (88.73%) samples that reacted to a single serovar, the serovar Canicola was considered to be the most likely in 89 cases (76.07%). Variables associated with the risk of disease were investigated using an epidemiological questionnaire administered to each owner, and the data obtained were analyzed using the statistical software EpiInfo. The significant variables (p ≤ 0.05) associated with seropositivity for leptospirosis in pet dogs in the urban area of the municipality of Jataizinho, PR, were the presence of rats in the household, adult or elderly dogs, free access of the dog to the street and contact with stray dogs. The results indicate a high prevalence of pet dogs in the urban area of Jataizinho, PR, with anti-leptospiral antibodies and suggest the need to educate the population of the municipality about the vaccination of dogs, as well as, the epidemiological role of the dog in urban leptospirosis. Key words: Epidemiology, microscopic agglutination, test, Leptospira spp., serovar Canicola, diagnosis, risk variables ResumoO estudo da prevalência de cães domiciliados em áreas urbanas com anticorpos antileptospiras e de variáveis associadas ao risco de infecção, pode auxiliar na identificação dos sorovares presentes na região e sugerir os reservatórios envolvidos nesta importante zoonose. O objetivo deste trabalho foi determinar a prevalência de anticorpos antileptospiras em 653 cães de 369 domicílios distribuídos na área urbana do município de Jataizinho, PR, e estudar variáveis associadas ao risco de infecção por leptospiras nesses animais. As amostras de soro foram submetidas a prova de soroaglutinação microscópica (SAM) frente a 23 sorovares de leptospiras patogênicas. Das 132 (20,21%) amostras de cães reagentes, 15 (11,36%) reagiram para mais de um sorovar, não sendo possível determinar o sorovar mais provável, e das 117 (88,73%) que reagiram para um sorovar somente, o sorovar Canicola foi considerado o mais provável em 89 (76,07%). Variáveis associadas ao risco da doença foram investigadas através de um questionário epidemiológico aplicado a cada proprietário e os dados obtidos foram analisados pelo programa estatístico EpiInfo. As variáveis signifi...

RESUMO.-[Caracterização molecular de DNA de Deltapapillomavirus bovino (BPV1, 2 e 13) em sarcoides equinos.] Sarcoides são tumores fibroblásticos, considerados os tumores de pele mais comuns em pele de equinos e que raramente apresentam regressão espontânea. Papilomavírus bovino (BPV) tipos 1 e 2 são relacionados com a patogenia do sarcoide e, provavelmente, o BPV tipo 13 (BPV13), recentemente descrito, também pode estar associado com a formação dessa lesão. Neste estudo, 20 amostras de lesões cutâneas, sendo 12 constituídas por tecidos frescos e 8 amostras de tecido fixado em formalina e embebido em parafina, provenientes de 15 cavalos foram utilizadas para a identificação do DNA de BPV. A análise histopatológica (HE) confirmou todas as lesões como sarcoide. Para a amplificação do DNA de papilomavírus (PV) foram realizadas três reações de PCR. Como triagem, os primers IFNR2/IDNT2 br Sarcoids are fibroblastic lesions, which are considered as the most common skin tumors of horses; spontaneous regression rarely occurs. The bovine papillomavirus (BPV) types 1 and 2 may be involved in the pathogenesis of sarcoids, and probably the recently described BPV type (BPV13) might be associated with the pathogenesis of this lesion. This study characterized the DNA of BPVs in sarcoids from 15 horses from Brazil by analyzing 20 cutaneous lesions (12 recently collected; 8 from formalin-fixed paraffin-embedded (FFPE) tissues). Histopathology confirmed the proliferative lesions as sarcoids. Three PCRs were performed to amplify papillomavirus (PV) DNA. For screening, the primers IFNR2/ IDNT2 were used to amplify a fragment of the PV L1 ORF. The second primer set was complementary to a common sequence of the E5L2 genomic region of BPV1, 2, and 13. The third primer pair (FAP59/FAP64) targeted a fragment of the PVs L1 ORF. The screening and E5L2 PCRs yielded amplicons in all samples evaluated. The FAP amplicons identified BPV1, 2, and 13 only from fresh tissue samples. The phylogenetic analyses of E5L2 resulted in the identification of BPV1, 2, and 13 in 14 (70%), 2 (10%), and 4 (20%) sarcoids, respectively. Two horses demonstrated multiple lesions: the sarcoids of one of these contained only BPV1 DNA and those of the other contained three types of bovine Deltapapillomavirus (BPV1, 2, and 13). This study confirmed the presence of BPV1, 2, and 13 DNA in equine sarcoids. Moreover, these findings represent the first description of three types of BPV diagnosed in the same horse, as well as the first confirmation of BPV1 and 2 in horses from Brazil.INDEX TERMS: Papillomavirus, Bovine Deltapapillomavirus, BPV1, 2, and 13, DNA, equine sarcoids, horse, skin tumor.