The optical effects of laser irradiation on whole human blood were studied. The normal blood samples were taken from healthy persons and the blood samples were exposed to laser beam. Three wavelengths of continuous wave (CW) for diode pumped solid state lasers (DPSSL S) were used to irradiate the blood samples. The wavelengths are (632 nm, 532 nm,473nm and405 nm ) and output varied between ( 3.9, 50,20 and 4.5) mW respectively. The laser exposure time fixed between (5 and 10 ) min for each wavelength. Laser radiation has a great role in the absorption properties of the normal human blood after being compered with absorption spectrum of a non-irradiated sample.

The blood of human has a number of optical characteristics resulting from irradiation by the beam of laser were studied. The samples of healthy blood were collimated of normal persons and persons with thalassemia, the sample of blood were exposed to laser beam. Continuous wave (CW) for diode pumped solid state lasers (DPSSL’S) were utilized in irradiate the samples of blood with wavelength (473 nm, 20 mW). The laser exposure time varied between (5 and10) min for each sample. Laser radiation has a great role in the absorption effects of the normal human blood after being compered with absorption spectrum of donors with thalassemia.