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The present study was undertaken to investigate the effects of two type of laser (532 nm with energy 4 mW and 650 nm with energy 135) on physical characteristics of serum proteins. All subjects of study were 15 men and their ages ranged between 18 to 25 years old. The blood samples were left to coagulate and centrifuged to obtained sera. Sera samples were divided in to three groups, the first group left without irradiation to serve as a control group, the second group was irradiated with laser 532 nm with 4mW for 10 minutes, and the third group was irradiated with laser 650 nm with 135 mW energy for 10 minutes. After irradiation, all samples were analyzed by using HPLC instrument. The results were indicated there were a significant changes among peaks of curves of irradiated samples compared to control group. Also, there were many obvious differences were noted among irradiated groups. From results mentioned above can be concluded that laser energy may be cause and create bonds inter and intra molecules of proteins across oxidation processes that finally change morphological and modifications structures of proteins molecules.

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