Aim:The aim of the present study was to evaluate the change in enamel structure and mineral content of artificially created white spot lesion treated either by chemical or Laser assisted bleaching preceded or followed by miswak application Materials and Methods: Miswak freezed dried extract was prepared from freshly cut chewing sticks. Ten human sound premolar teeth freshly extracted due to orthodontic causes were divided into two halves in a mesio-distal direction two halves. A total of twenty specimens were obtained and undergone baseline surface scanning by EDX/SEM. White spot lesion is first artificially created by immersing specimens in demineralizing solution for five days. Another enamel surface scanning by EDX/SEM were done for all specimens after demineralization. The specimens were either undergone chemical bleaching using 36% hydrogen peroxide alone or Laser assisted bleaching that is performed by same concentration hydrogen peroxide activated by diode low energy Laser 980 nm. Miswak is either placed on white spot lesion before bleaching or after bleaching. A final EDX/ SEM is done after treatment.Results: For chemical bleaching group, placement of miswak on wsls followed by chemical bleaching results in enhanced remineralization with significant increase in calcium and phosphate of bleached enamel. For Laser bleaching group, placing miswak on wsls after laser activated bleaching results in significant increase in phosphate level of bleached enamel in addition to enhanced remineralization. Conclusion:Miswak can remineralize white spot lesion treated with chemical or Laser bleaching technique with proper protocol.