Objective:The objective of the present study was to evaluate the regenerative power of sticky bone in guided tissue regeneration alone compared to sticky bone with different biomodulation techniques in terms of bone density at the regenerated site.Materials and methods: 40 rats were randomly assigned into 5 groups (8 rats each), the control group (CG), the sticky bone group (SB), the sticky bone with LASER (SB-L), the sticky bone with PRF membrane, (SB-P), and sticky bone with LASER and PRF (SB-L-P). A rectangle shaped bone defect (3ml height and 4 mml length) was created in the diastema between the incisors and molar area with preservation of the lingual plate of bone at the mandible of all rats. The defects were filled according to the assigned test groups. 60 days after surgery the rats were radiographically examined to obtain the bone density measurements. Results:The measurement of bone density in the defect has shown that the SB-L group and SB group had the highest mean (172.5± 15), (169.9± 14.9) respectively. The difference between the two groups were not statistically significant. However, both groups were statistically significant when compared to all three groups, CG, SB-P group and SB-L-P group. All three groups had the lowest mean (72.5±4.7), (74.1±8.4), (88.2±14.5). There were no statistically significant differences between these three groups. Conclusions:The sticky bone augmented with LASER biomodulation has demonstrated higher bone density compared to sticky bone alone as graft material. However, the addition of PRF, or LASER and PRF has shown to be non-significant.