Leptospirosis is an infectious disease affect human and animal. The diagnosis is challenging due to none specific clinical features, lack of awareness and poor access to health care. Therefore the disease becomes underreported. Early diagnosis is essential because antibiotic treatment is most effective when it is initiated early. However, laboratories tests for early diagnosis still lacking. The disease may be demonstrated by direct or indirect methods. Direct methods are the detection of the organism or its components and indirect methods are the detection of specific antibodies of the organism. This review highlights the major diagnostic techniques in leptospirosis with their advantages and disadvantages as well as the treatment. The search criteria focused on the thesis we obtained and searching in the websites (Google

Background: Currently, qPCR has been used as a rapid diagnostic method for human leptospirosis. Previous studies have indicated that qPCR has high sensitivity in the early days of the illness. Objectives: The aim of this study was to evaluate qPCR as a diagnostic method for human leptospirosis in the National Institute of Hygiene, Rabat, Morocco. Methods: From 2004 to 2016, 67 sera related to 67 patients with clinical signs mimic to leptospirosis were sent to the laboratory of Bacteriology for routine diagnosis and confirmation. SAT, ELISA IgM, ELISA IgG, and qPCR were used for the diagnosis. Results: High positivity was observed by SAT (88.24%), ELISA IgM (58.82%), and real-time PCR (17.64%), in sequence. No negative results by serological tests had positive results by real-time PCR. Forty-six patients were males (68.68%) and 21 were females (31.34%). The high incidence observed was from Sidi Qacem (40%). Conclusions: SAT and ELISA IgM are useful for the diagnosis of human leptospirosis in Morocco and they can provide prompt and low-cost diagnosis, especially when resources are limited.