The study of genetic diversity and phenotypic variability in the available germplasm is a prelude to crop improvement. The present study was undertaken to characterize 10 accessions of Capsicum annuum acquired from the Centre for Genetic Resources, the Netherlands (CGN) through Plant Genetic Resources Institute (PGRI), National Agricultural Research Centre (NARC) Islamabad. The accessions were evaluated for 35 qualitative and 11 quantitative parameters. Wide variation was noted among the genotypes for important characters pertaining to fruit and seed yield. Such genetic variability can be a valuable resource for genetic improvement including developing resistance against insect pests and diseases. The available accessions need to be characterized in further detail under Pakistani agro-climatic conditions to ascertain the statistical significance of variability, understand the genetic control and gene interactions involved.