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Sutter, Vera L., and Carter, Walker T.: Evaluation of media and reagents for indole-spot tests in anaerobic bacteriology. Am. J. Clin. Pathol. 58: 335-338, 1972. Indole-spot tests for use in anaerobic bacteriology were evaluated. Spot test results obtained with four reagents and 143 anaerobic organisms grown on blood agars prepared with three commonly-used bases were compared with those obtained with a tube test utilizing indole-nitrate medium and Kovacs' test. Correlation of spot test with tube test results was best when organisms were grown on trypticase soy blood agar and tested with 1% p-dimethylaminocinnamaldehyde in 10% (v/v) concentrated hydrochloric acid. This indole-spot test offers a simple, reliable, economical, and rapid method for detection of indole production with anaerobic bacteria.

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