Abbreviations: AIBN, azobisisobutyronitrile; CE-LIF, capillary electrophoresis laser-induced fluorescence detection; EDMA, ethylene dimethacrylate; GC-MS, gas chromatography-mass spectrometry; MISPE, molecularly imprinted solid-phase extraction; HPLC, high-performance liquid chromatography; LOD, limit of detection; LOQ, limit of quantitation; MIP, molecularly imprinted polymers; MAA, methacrylic acid; NIP, non-imprinted polymer; NISPE, non-imprinted solid-phase extraction; RSD, relative standard deviation; SEM, scanning electron microscopy; SPE, solid-phase extraction Keywords: Brown sugar/Capillary electrophoresis/In situ polymerization/MIPs/ Monolithic capillary column/Rose bengal/SPE Abstract 1 A method employing molecularly imprinted solid-phase extraction (MISPE) coupled 2 with capillary electrophoresis laser-induced fluorescence detection (CE-LIF) was 3 developed for the determination of rose bengal in brown sugar. The rose bengal 4 imprinted monolithic column was prepared by in situ polymerization, utilizing rose 5 bengal as template, methacrylic acid as functional monomer and ethylene 6 dimethacrylate as cross-linker. The capacity and specificity of this column were 7 evaluated by CE-LIF, and the morphology was characterized by scanning electron 8microscopy. The recoveries were ranging from 89.7% to 90.3% (relative standard 9 deviation 3.6%-4.5%, n=5). The capacities of molecularly imprinted polymer and 10 non-imprinted polymer columns were 1.314 µg mg -1 and 0.531 µg mg -1 , respectively. 11The detection limit (S/N=3) was 3 ng mL -1 . The enrichment factor was higher than 12 63-fold and the MISPE column managed to clean up the matrix in brown sugar 13 effectively. This method can be applied to sensitively and effectively determine rose 14 bengal in brown sugar.