We introduce topological theory of perfect isolation: perfect transmission from one side and total reflection from another side simultaneously. The theory provides an efficient approach for determining whether such a perfect isolation point exists within a finite parameter space. Herein, we demonstrate the theory using an example of a Lorentz non-reciprocal metasurface composed of dimer unit cells. Our theory also suggests that perfect isolation points can annihilate each other through the coalescence of opposite topological charges. Our findings could lead to novel designs for high-performance optical isolators.

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