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This study aimed to determine the effect and comparison of histopathological features of the skin of domestic cat (Felis domestica) on the formation of collagen fibers in the incise wound after treated by sugar and honey. Materials and methods in this study 15 male domestic cats were used in this study and were divided into five groups of Negative control group (KKN) without treatment, Positive control group (KKP) used Bioplacenton®, Group 1 (KP1) used sugar, Group 2 (KP2) used honey and Group 3 (KP3) used sugar and honey (combination) for 14 days, topically. The incision wound was made on the back of the domestic cat and the treatments were given daily for 14 days; the skin samples were taken on days 4, 9 and 14 to observe the formation of collagen fibers. The process of collagen fibers formation was observed and scoring was done from score 0 to +4 based on the density of collagen fibers in the wound area. Score changes data were analyzed descriptively. Results showed that KKN and KP2 collagen fibrous density was similar at day 4 score 0, day 9 score +1, and day 14 score +2. KP1 and KP3 collagen fibrous density were similar at day 4 score +1, day 9 score +2, and day 14 score +3. While KKP collagen fibrous density was high at day 4 score+1, day 9 score +3, and day 14 score +4. KP3 showed a good collagen fiber formation with the density was almost similar to the positive control group (KKP). The control groups (KKN and KKP) and the treatment groups (KP1, KP2 and KP3) showed differences in the formation of collagen fibers, fibroblast cells and the discovery of inflammatory cells on days 4, 9 and 14 during the wound healing process. The provision of sugar and honey in combination showed a good influence in the process of formation of collagen fibers during the wound healing process.

This study aimed to observed the fibroblast cell description in incision wound of the domestic cat after treated by sugar and honey. Fifteen domestic cats (Felis domestica) were used in the research. The cats were divided into five treatment groups as follow, the negative control group (KK1) was normal wound healing (without any treatment), the positive control group (KK2) used Bioplacenton® ointment, group 1 (KP1) used sugar, group 2 (KP2) used honey, and group 3 (KP3) used a combination of sugar and honey. All preparations were given topically for 14 days. The sample collection of the skin was performed on the 4th, 9th, and 14th days. Histopathological preparation with hematoxylin-eosin staining was conducted on all skin samples. Treatment with a combination of sugar and honey showed a very high increase in fibroblast cells, and this also occurred in KP1, KK2 and KK1. Group treated with honey (KP2) showed low fibroblast cell level. This research revealed that the combination of sugar and honey showed a good influence on the process of fibroblast cell formation during the wound healing process.