An experiment to investigate "The response of varieties to air-layering in Olive cultivars" was conducted at Olive Research Farm Sangbatti, Agricultural Research Institute (ARI) Tarnab, Peshawar, Pakistan during july 2016. Air layering was done in June Olive cultivars i-e Local variety, Leccino variety, Pendolino variety, Coratino variety and chetoui variety were compared. The experiment was laid out on a single factor RCB-Design (randomized complete block design) having three replications. Maximum root diameter (1.42 mm), number of roots plant -1 (6), Minimum days to callus formation (20days) and rooting (55 days) were recorded for Leccino. And maximum root length (5.56cm) and weight (0.67gm) were recorded for local variety. While minimum root length (1.5cmcm), root diameter (1.13mm), root weight (0.17gm) and number of roots plant -1 (1.66), Maximum days to callus formation (27days) and rooting (68days) were recorded for coratina. On the basis of these results it was concluded that Leccino variety performed best in response to days to callus formation, rooting, diameter and number of roots through air layering in different Olive cultivars.