Gleditsia triacanthos L. is a deciduous tree belonging to the family Fabaceae. It possesses important biological activities as anti-mutagenic, anticancer, cytotoxic and treating rheumatoid arthritis. The total ethanol extract (EtOHE) and successive extracts (petroleum ether, chloroform, ethyl acetate, and aqueous ethanol) were prepared from the leaves. Eight flavone glycosides and two flavone aglycones named vicenin-I (1), vitexin (2), isovitexin (3), orientin (4), isoorientin (5), luteolin-7-O-ß-glucopyranoside (6), luteolin-7-O-ß-galactopyranoside (7), apigenin-7-O-ß-glucopyranoside (8), luteolin (9) and apigenin (10) were isolated from the aqueous ethanol extract of G. triacanthos L. leaves. Potent cytotoxic activity of the EtOHE extract was observed against the liver (IC50 = 1.68 μg), breast (IC50 = 0.74 μg), cervix (IC50 = 1.28 μg), larynx (IC50 = 0.67 μg) and colon (IC50 = 2.50 μg) cancer cell lines. Cytotoxic activity of compounds 2, 4, 6 and 8 against, the liver, breast and colon cancer cell lines was also proved. Evaluation of the in-vivo antioxidant activity of the EtOHE and successive extracts revealed that the highest activity was exhibited by 100 mg of EtOHE (97.89% potency) as compared with vitamin E (100% potency). Compound 6 showed 91.8% free radical scavenging activity.

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Saraca indica L. (Saraca asoca) is reported for its important biological benefits in Indian folk medicine. The powdered leaves were extracted with 70% ethanol to obtain total ethanol extract(TEE) that was successively fractionated to get petroleum ether(pet. ether),chloroform(CHCl3), ethyl acetate (EtOAc) and methanol extracts. The antioxidant activity of TEE by three methods of assay, as well as, the antioxidant activity of TEE and successive extracts by DPPH method at two dose were done.Total phenolics and total flavonoids content of the plant were determined. The cytotoxic evaluation revealed that EtOAc extract exhibited activity against HCT-116 andMCF-7, Hela& HEPG-2 . GC/MS analysis of the unasponifiable(USF) and saponifiable (SF)fractions of the non-polar extract was carried out. 34 Compounds with phytol, dodecane, BHT and α -amyrin as the major compounds in the USF and 25 compounds with methyl palmitate & Methyl-9, 12,15-octadecatrienoate as major compounds in the SF were identified. Fifteen phenolic compounds were detected in HPLC analysis of EtOAcextract as well as six phenolic compounds were isolated and identified for the first time from the same extract they were; gallic acid, methylgallate, rutin, quercetin -3-O-rhamnoside, quercetin and kaempeferol.

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