In this study, detection of 20 FITC-derivatized amino acids using an MEEKC-LIF was demonstrated. In order to achieve good separation for hydrophobic amino acids, the MEEKC method was employed and detection limits were obtained in the range of 0.32-2.2 nM, which is comparable to previous reports on amino acid analyses. Furthermore, a significant reduction in the reaction time from 1 h for conventional derivatization to 3 min for the microwave-assisted derivatization was observed and achieved, as opposed to the traditional pretreatment of real sample due to its complexity prior to the analysis of amino acid content. Finally, this microwave-assisted derivatization MEEKC-LIF method successfully determined amino acids in beverage, food, and biological samples (rat brain) with good recovery.

ＤＯＩ： １０．３７２４ ／ ＳＰ．Ｊ．１１２３．２０１８．０６０２５ * 收稿日期：２０１８•０７•０２ * 通讯联系人．Ｔｅｌ：(０２８)８７８７７１４２，Ｅ•ｍａｉｌ：１１３３４３８３８＠ ｑｑ．ｃｏｍ． 基金项目：" 食品安全关键技术研发" 重点专项(２０１７ＹＦＣ１６０１３００) ；国家食品药品监督管理总局研究项目． Ｆｏｕｎｄａｔｉｏｎ ｉｔｅｍ： Ｎａｔｉｏｎａｌ Ｓｐｅｃｉａｌ Ｐｒｏｊｅｃｔ ｆｏｒ " Ｋｅｙ Ｓｃｉｅｎｃｅ ａｎｄ Ｔｅｃｈｎｏｌｏｇｙ ｆｏｒ Ｆｏｏｄ Ｓａｆｅｔｙ " (Ｎｏ． ２０１７ＹＦＣ１６０１３００) ； Ｒｅｓｅａｒｃｈ Ｐｒｏｊｅｃｔ ｏｆ Ｃｈｉｎａ Ｆｏｏｄ ａｎｄ Ｄｒｕｇ Ａｄｍｉｎｉｓｔｒａｔｉｏｎ．

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