The silica gel surface was alkylated by silane coupling agent KH-570 under the microwave assisted and orthogonal experiment was adopted to optimize the silica gel surface silanized experimental conditions with synthetic yield as index. With quercetin as the template molecule, 4 - vinyl pyridine as the functional monomer, ethylene glycol dimethyl acrylate as the cross linking agent, azodiisobutyronitrile as initiator, under the microwave assisted in silane silicone surface quercetin molecularly imprinted polymer was successfully synthesized. Take selected quercetin as index, another orthogonal experiment was implemented to optimize the quercetin-selective MIPs synthesis experiment on silica gel surface. What is more, the adsorptive property of quercetin-selective MIPs which was grafted on silica gel surface has been inspected. We have extracted of quercetin in the ethyl acetate from raspberry with quercetin-selective MIPs grafted on silica gel surface. Synthetic time have been shortened more than a dozen times, the efficiency of preparation of MIPs have been greatly improved.

A method was estimated to detect pentachlorophenol and sodium pentachlorophenolate in fishery products by gas chromatography with μ-ECD detector. Procedures of the method included extraction, alkaline stripping and derivatization. The pH of sample matrix was modified to 3-4 by nitric acid solution(nitric acid:water 1:1). Target compounds were extracted by hexane first and 0.2mol/L potassium hydroxide solution was used as stripping reagent to isolate pentachlorophenol from hexane. Acetic anhydride was taken as derivatizing reagent to convert target compounds into nonpolar ester compounds according to acylation reaction. Derivatized compound was extracted by hexane for the analysis of GC. The limit of detection is 2µg/kg . The average recoveries ranged from 73.01 to 104.68% spiked at 2.0,4.0,10.0µg/kg. And the relative standard deviations ranged from1.5 to 8.3%. The method can be used for the determination of pentachlorophenol and sodium pentachlorophenolate in fishery products,and it provides an rapid and acute method for food safety determination.