No abstract

No abstract

A new continuous affinity chromatography for enzyme purification and concentration was proposed. It combined the features of pH-parametric pumping and simulated moving bed operations. Adsorption and desorption were taking place simultaneously in the system. Regeneration of adsorbent was performed by successively shifting the adsorption and desorption sections from one column to another with a time controller. Performance tests for enzyme separation and purification were carried out by devising six-staged affinity chromatographic columns, 16cm ID x 40cm Ht, packed with lOcm height chitin-CHOM each for trypsin purification. Factors affecting the successful operations of the systems and separation efficiency of enzyme were investigated. The system was applied to the purification of trypsin from extract of porcine pancreas. It was found that fairly high separation efficiency can be achieved, SAR (specific activity ratio = specific activity of productlspecific activity of feed) of eluant was as high as 3.9. Thus it is considered as a promising method of enzyme purification. For purification of c~d e enzyme, SMB (Simulated Moving Bed) can be incorporated with a washing section between the adsorption and desorption sections for washing out the impure proteins contaminated into the columns.