Bone defects created after curettage of benign bone tumors are customarily filled with solid poly(methyl methacrylate) (PMMA) or other bone substitutes. In this study, we depicted a porous PMMA-based cement (produced by mixing sodium bicarbonate and citric acid) and evaluated the prospect of its clinic application. Cement samples were characterized by high-performance liquid chromatography (HPLC) coupled to mass spectrometry and its cytotoxicity evaluated in fibroblast cultures. Implantation in rabbits allowed the histologic analysis of bone, kidneys, and liver for toxicity and coagulation tests, and MRI images for hemostasis evaluation. Osseointegration was analyzed through radiography, microtomography (micro-CT), SEM, and histology of sheep specimens. Rabbit specimens were analyzed 1, 4, and 7 days after implantation of porous or solid bone cement in 6.0 mm femoral defects. Sheep specimens were analyzed 3 and 6 months after implantation or not of porous or solid cement in 15.0 mm subchondral tibial defects. The production process did not release any detectable toxic substance but slightly reduced fibroblast proliferation in vitro. Until 7 days after surgery, no local or systemic alterations could be detected in histology, or hematoma formation in histology or MRI. Sheep implants showed 6 mm linear ingrowth from the bone-cement interface and 20% bone ingrowth considering the whole defect area. Radiography, micro-CT, SEM, and histology confirmed these findings. We conclude that our porous PMMA-based cement is an attractive alternative treatment for bone defect filling that combines osseointegration and early weight bearing. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 649-658, 2018.

A acalasia cricofaríngea é uma doença caracterizada pela interrupção da passagem dos alimentos através do esfíncter esofágico cranial, condição essa associada com a incapacidade do relaxamento muscular durante a deglutição cricofaríngea ou com as descoordenadas contrações dos músculos da faringe. Um cão, fêmea, Golden Retriever, seis meses de idade, que apresentava regurgitação, foi avaliado. Na radiografia contrastada do esôfago cervical foi visibilizada a presença do meio de contraste na laringe e no esôfago, no momento da deglutição, e quantidade discreta de bário na traqueia proximal. Após exame radiográfico, o animal foi submetido a ato operatório sendo constatada melhora no quadro clínico. Embora o melhor método de avaliação por imagem seja a fluoroscopia, o objetivo do presente relato de caso é o de discorrer sobre o diagnóstico positivo para acalasia cricofaríngea mediante exame radiográfico contrastado.

Background: In dogs pheochromocytoma usually compress and invade into the adjacent vessels and structures, which may lead to the occurrence of caudal vena caval tumor thrombi. The surgical approach in these cases requires adrenalectomy with caval thrombectomy. Conventionally, tourniquets and partial occlusion clamp are used to reduce intraoperative blood loss, but in dogs with a large thrombus associated to a great local tumor invasion, the traditional technique may be hindered. The aim of this study was to report the use of a purse-string suture during venotomy of the caudal vena cava for thrombectomy, as an alternative to tourniquet and partial occlusion clamp.Case: An 11-year-old male Daschound breed dog, was referred for evaluation because of the appearance of small blackened nodules in the ventral abdomen. During a search for metastatic lesions with abdominal ultrasound, the right adrenal was identified with significant volume increase, and presence of tumor-associate circular formation that appeared to invade the caudal vena cava, suggestive of neoplastic invasion or thrombus. Abdominal computed tomography showed caudal vena cava in its hepatic portion presenting considerable increase in diameter, with caudal dilation and presence of hypoattenuating material in its lumen. Right adrenal gland with rounded appearance and regular borders, heterogeneous parenchyma and juxtaposed caudal vena cava suggesting invasion of this, confirming the sonographic findings. During exploratory celiotomy, adherence of the adrenal mass to the right renal vessels and invasion of the caudal vena cava were observed, leading to the need of nephrectomy and venotomy for thrombectomy. To perform the venotomy, a Rumel tourniquet was placed loosely around the vena cava only caudal to the invasion point of the tumor thrombus, cranially, tourniquet application was not possible due to the presence of a large thrombus extending into the intrahepatic cava. Therefore, a purse-string suture was applied around the invasion point of the vena cava tumor thrombi. Thus, during the thrombectomy, while dissecting with the scalpel blade, the purse-string suture was gently tightened, minimizing hemorrhage and allowing the venotomy to close immediately after thrombi removal. Histopathological mass analysis allowed the diagnosis of malignant pheochromocytoma with intravascular extension of the tumor, and the patient remained without clinical changes for 18 months.Discussion: When caval invasion by the adrenal mass is present, the application of Rumel tourniquets around the vena cava cranial and caudal to the tumor thrombus invasion point is recommended to reduce intraoperative hemorrhage during thrombectomy. However, due to the presence of a large thrombus extending into the intrahepatic cava in the presente report, it was not possible to apply the cranial tourniquet, just the caudal one. Thus, the technique adapted using a purse-string suture during venotomy of the caudal vena cava for thrombectomy was extremely efficient in controlling hemorrhage, while allowing rapid venotomy closure without the need for partial occlusion vascular clamp, reducing the surgical time. A tumor thrombus in the vena cava associated with adrenal gland tumors are amenable to adrenalectomy and thrombectomy without significantly increasing perioperative morbidity and mortality rates, assuming the surgeon is experienced in appropriate techniques. Moreover, the presence of tumor invasion does not seem to be predictive of the outcome of the cases, in agreement with what happened in this case.