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a b s t r a c tA number of approaches have been proposed for screening level ecological risk assessment. In this paper, we first established a mass spectrum library including 50 phenols using retention time locking (RTL) technology and deconvolution reporting software (DRS). Distribution of phenols in surface water of the Taihu Lake was screened. Among the 22 identified phenols, 14 phenols were quantified. The concentrations of total phenols ranged 675.2-3346.1 ng L À1. The distributions of ecological effect quotients (EEQs) of 14 phenols were characterized in terms of the exposure concentration distributions (ECDs) and species sensitivity distributions (SSDs). Those phenols with EEQs exceeding the threshold proposed by Water Environment Research Foundation of Alexandria were selected as priorities. As a result, 2-nitrophenol (2-NP), p-chloro-m-xylenol (PCMX) and pyrocatechol were determined as potential ecological risk stressors in surface water of the Taihu Lake. Results of the present study suggested that the proposed approach is feasible for the screening level ecological risk assessment.