No abstract

In this paper, we employed the rice variety Wanlun 422 with wide compatibility, WA-type restorer line Shuhui 527, Miyang 46, and Miyang 42 as the parent materials, and selected the Japonica rice restorer line for Yinshui type cytoplasmic male sterility (YS-CMS) from the hybrid combinations F 2 and F 4 using molecular marker-assisted selection combined with the conventional breeding method. The results showed that the plants containing two restorer genes identified by molecular markers were selected to make hybrid combinations with YS-CMS lines. We found that the seed-setting rate and heterosis of the hybrids were significantly improved. The results of this paper indicate that it is feasible for development of restorers for Japonica YS-CMS line based on molecular marker-assisted.